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Yorodumi- PDB-1nzr: CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUD... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1nzr | ||||||
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Title | CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION | ||||||
Components | AZURIN | ||||||
Keywords | ELECTRON TRANSPORT (COPPER) | ||||||
Function / homology | Function and homology information transition metal ion binding / periplasmic space / electron transfer activity / copper ion binding / zinc ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.2 Å | ||||||
Authors | Tsai, L.-C. / Sjolin, L. / Langer, V. / Bonander, N. / Karlsson, B.G. / Vanngard, T. / Hammann, C. / Nar, H. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1995 Title: Structure of the azurin mutant nickel-Trp48Met from Pseudomonas aeruginosa at 2.2 A resolution. Authors: Tsai, L.C. / Sjolin, L. / Langer, V. / Bonander, N. / Karlsson, B.G. / Vanngard, T. / Hammann, C. / Nar, H. #1: Journal: J.Mol.Biol. / Year: 1991 Title: X-Ray Crystal Structure of the Two Site-Specific Mutants His35Gln and His35Leu of Azurin from Pseudomonas Aeruginosa Authors: Nar, H. / Messerschmidt, A. / Huber, R. / Van De Kamp, M. / Canters, G.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1nzr.cif.gz | 142.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1nzr.ent.gz | 113.3 KB | Display | PDB format |
PDBx/mmJSON format | 1nzr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1nzr_validation.pdf.gz | 396.3 KB | Display | wwPDB validaton report |
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Full document | 1nzr_full_validation.pdf.gz | 403.2 KB | Display | |
Data in XML | 1nzr_validation.xml.gz | 11.2 KB | Display | |
Data in CIF | 1nzr_validation.cif.gz | 19.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nz/1nzr ftp://data.pdbj.org/pub/pdb/validation_reports/nz/1nzr | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 13906.784 Da / Num. of mol.: 4 / Mutation: W48M Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / References: UniProt: P00282 #2: Chemical | ChemComp-NI / #3: Chemical | ChemComp-NO3 / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.22 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 297-298 K / pH: 5.7 / Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Num. obs: 17974 / % possible obs: 73 % / Num. measured all: 82349 / Rmerge(I) obs: 0.087 |
-Processing
Software |
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Refinement | Rfactor Rwork: 0.17 / Rfactor obs: 0.17 / Highest resolution: 2.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.2 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 8 Å / Num. reflection obs: 17394 / Rfactor obs: 0.17 / Rfactor Rwork: 0.17 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |