+Open data
-Basic information
Entry | Database: PDB / ID: 1e5y | ||||||
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Title | Azurin from Pseudomonas aeruginosa, reduced form, pH 5.5 | ||||||
Components | AZURIN | ||||||
Keywords | ELECTRON TRANSPORT / COPPER BINDING | ||||||
Function / homology | Function and homology information transition metal ion binding / electron transfer activity / periplasmic space / copper ion binding / zinc ion binding / identical protein binding Similarity search - Function | ||||||
Biological species | PSEUDOMONAS AERUGINOSA (bacteria) | ||||||
Method | X-RAY DIFFRACTION / OTHER / Resolution: 2 Å | ||||||
Authors | Nar, H. / Messerschmidt, A. | ||||||
Citation | Journal: Ph.D. Thesis / Year: 1992 Title: Rontgenkristallographische Strukturaufklarung Von Azurin Aus Pseudomonas Aeruginosa Authors: Nar, H. #1: Journal: J.Mol.Biol. / Year: 1991 Title: Crystal Structure Analysis of Oxidized Pseudomonas Aeruginosa Azurin at Ph 5.5 And Ph 9.0 Authors: Nar, H. / Messerschmidt, A. / Huber, R. / Van De Kamp, M. / Canters, G.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1e5y.cif.gz | 109.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1e5y.ent.gz | 90 KB | Display | PDB format |
PDBx/mmJSON format | 1e5y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e5/1e5y ftp://data.pdbj.org/pub/pdb/validation_reports/e5/1e5y | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given / Matrix: (1), |
-Components
#1: Protein | Mass: 13961.799 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PSEUDOMONAS AERUGINOSA (bacteria) / Cell line (production host): KMBL1164 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): K-12 / References: UniProt: P00282 #2: Chemical | ChemComp-CU / #3: Chemical | ChemComp-NO3 / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.79 % |
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Crystal grow | pH: 5.5 / Details: PH 5.5 |
-Data collection
Diffraction | Mean temperature: 288 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: BRUKER NONIUS FAST / Detector: AREA DETECTOR / Date: Mar 15, 1991 |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.98→50 Å / Num. obs: 29834 / % possible obs: 77 % / Observed criterion σ(I): 2.5 / Rmerge(I) obs: 0.08 / Rsym value: 0.078 |
Reflection shell | Resolution: 1.98→2.05 Å / % possible all: 29.4 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 22.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.22 Å / Luzzati d res low obs: 8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.1 Å / Rfactor Rwork: 0.28 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file | Serial no: 1 / Param file: PARA19X.PRO / Topol file: TOPH19X.PRO |