- PDB-2atf: X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS ... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2atf
Title
X-RAY STRUCTURE OF cysteine dioxygenase type I FROM MUS MUSCULUS MM.241056
Components
Cysteine dioxygenase type I
Keywords
OXIDOREDUCTASE / Mm.241056 / pfam05995.2 CDO_I / BC013638 / CUPIN FAMILY / STRUCTURAL GENOMICS / PROTEIN STRUCTURE INITIATIVE / PSI / Center for Eukaryotic Structural Genomics / CESG
Function / homology
Function and homology information
L-cysteine catabolic process to taurine / Degradation of cysteine and homocysteine / taurine metabolic process / taurine biosynthetic process / cysteine dioxygenase / cysteine dioxygenase activity / L-cysteine catabolic process / response to glucagon / nickel cation binding / response to amino acid ...L-cysteine catabolic process to taurine / Degradation of cysteine and homocysteine / taurine metabolic process / taurine biosynthetic process / cysteine dioxygenase / cysteine dioxygenase activity / L-cysteine catabolic process / response to glucagon / nickel cation binding / response to amino acid / response to glucocorticoid / response to cAMP / response to organonitrogen compound / lactation / ferrous iron binding / response to ethanol / zinc ion binding / plasma membrane / cytosol Similarity search - Function
Cysteine dioxygenase type I / Cysteine dioxygenase type I / RmlC-like cupin domain superfamily / Jelly Rolls / RmlC-like jelly roll fold / Jelly Rolls / Sandwich / Mainly Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 188 / Source method: isolated from a natural source / Formula: H2O
Nonpolymer details
The metal ion in this structure is currently modeled as Ni2+.ICP analysis indicates that there is a ...The metal ion in this structure is currently modeled as Ni2+.ICP analysis indicates that there is a mixture of metals in the protein sample, with Ni dominant, Zn next most abundant, and some Fe. The biologically relevant metal ion is expected to be Fe2+.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.2 Å3/Da / Density % sol: 43.6 %
Crystal grow
Temperature: 273 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 10 MG/ML PROTEIN, 20% MPEG 5K, 0.16 M CALCIUM CHLORIDE, 0.10 M MES, pH 6.0, vapor diffusion, hanging drop, temperature 273K