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Open data
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Basic information
Entry | Database: PDB / ID: 2ask | ||||||
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Title | Structure of human Artemin | ||||||
![]() | artemin | ||||||
![]() | HORMONE/GROWTH FACTOR / Glial cell derived family ligand / neurotrphoic growth factor / sulfates / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | ![]() glial cell-derived neurotrophic factor receptor binding / Peyer's patch morphogenesis / glial cell-derived neurotrophic factor receptor signaling pathway / lymphocyte migration into lymphoid organs / peripheral nervous system development / induction of positive chemotaxis / NCAM1 interactions / RET signaling / neuroblast proliferation / axon guidance ...glial cell-derived neurotrophic factor receptor binding / Peyer's patch morphogenesis / glial cell-derived neurotrophic factor receptor signaling pathway / lymphocyte migration into lymphoid organs / peripheral nervous system development / induction of positive chemotaxis / NCAM1 interactions / RET signaling / neuroblast proliferation / axon guidance / growth factor activity / receptor tyrosine kinase binding / RAF/MAP kinase cascade / signaling receptor binding / signal transduction / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Silvian, L. / Jin, P. / Carmillo, P. / Boriack-Sjodin, P.A. / Pelletier, C. / Rushe, M. / Gong, B.J. / Sah, D. / Pepinsky, B. / Rossomando, A. | ||||||
![]() | ![]() Title: Artemin crystal structure reveals insights into heparan sulfate binding. Authors: Silvian, L. / Jin, P. / Carmillo, P. / Boriack-Sjodin, P.A. / Pelletier, C. / Rushe, M. / Gong, B.J. / Sah, D. / Pepinsky, B. / Rossomando, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 52.3 KB | Display | ![]() |
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PDB format | ![]() | 40.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11984.789 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.31 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Magnesium Sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
-Data collection
Diffraction |
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 1, 2001 | ||||||||||||||||||
Radiation |
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Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||
Reflection | Resolution: 1.55→50 Å / Num. all: 30142 / Num. obs: 29225 / % possible obs: 96.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 3 / Redundancy: 3.2 % / Biso Wilson estimate: 21.6 Å2 / Rmerge(I) obs: 0.064 / Rsym value: 0.064 / Net I/σ(I): 19.3 | ||||||||||||||||||
Reflection shell | Highest resolution: 1.55 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 2.9 / Num. unique all: 2719 / Rsym value: 0.31 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 60.8269 Å2 / ksol: 0.425408 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.55→15.6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.55→1.65 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 6
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Xplor file |
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