+Open data
-Basic information
Entry | Database: PDB / ID: 2alr | |||||||||
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Title | ALDEHYDE REDUCTASE | |||||||||
Components | ALDEHYDE REDUCTASE | |||||||||
Keywords | OXIDOREDUCTASE / TIM-BARREL | |||||||||
Function / homology | Function and homology information glucuronate reductase / glucuronolactone reductase / glucuronolactone reductase activity / D-glucuronate catabolic process to D-xylulose 5-phosphate / Formation of xylulose-5-phosphate / alcohol dehydrogenase (NADP+) / D-glucuronate catabolic process / aldehyde catabolic process / alcohol dehydrogenase (NADP+) activity / cellular aldehyde metabolic process ...glucuronate reductase / glucuronolactone reductase / glucuronolactone reductase activity / D-glucuronate catabolic process to D-xylulose 5-phosphate / Formation of xylulose-5-phosphate / alcohol dehydrogenase (NADP+) / D-glucuronate catabolic process / aldehyde catabolic process / alcohol dehydrogenase (NADP+) activity / cellular aldehyde metabolic process / cellular detoxification of aldehyde / glutathione derivative biosynthetic process / aldo-keto reductase (NADPH) activity / L-glucuronate reductase activity / D/L-glyceraldehyde reductase / Glutathione conjugation / allyl-alcohol dehydrogenase / allyl-alcohol dehydrogenase activity / L-ascorbic acid biosynthetic process / daunorubicin metabolic process / doxorubicin metabolic process / aldose reductase (NADPH) activity / glucose metabolic process / oxidoreductase activity / electron transfer activity / apical plasma membrane / extracellular space / extracellular exosome / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.48 Å | |||||||||
Authors | El-Kabbani, O. | |||||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1994 Title: Structures of human and porcine aldehyde reductase: an enzyme implicated in diabetic complications. Authors: El-Kabbani, O. / Green, N.C. / Lin, G. / Carson, M. / Narayana, S.V. / Moore, K.M. / Flynn, T.G. / DeLucas, L.J. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1993 Title: Crystallization and Preliminary Structure Determination of Porcine Aldehyde Reductase from Two Crystal Forms Authors: El-Kabbani, O. / Lin, G. / Narayana, S.V.L. / Moore, K.M. / Green, N.C. / Flynn, T.G. / Delucas, L.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2alr.cif.gz | 69.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2alr.ent.gz | 52.6 KB | Display | PDB format |
PDBx/mmJSON format | 2alr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2alr_validation.pdf.gz | 369.8 KB | Display | wwPDB validaton report |
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Full document | 2alr_full_validation.pdf.gz | 380.5 KB | Display | |
Data in XML | 2alr_validation.xml.gz | 8.7 KB | Display | |
Data in CIF | 2alr_validation.cif.gz | 12.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/al/2alr ftp://data.pdbj.org/pub/pdb/validation_reports/al/2alr | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36486.770 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Organ: KIDNEY / Tissue: MEDULLA, CORTEX / References: UniProt: P14550, alcohol dehydrogenase (NADP+) |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 43 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.2 / Details: pH 6.2 | |||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 293 K / pH: 6.4 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Apr 14, 1992 / Details: COLLIMATOR |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 10591 / % possible obs: 92 % / Observed criterion σ(I): 0 / Redundancy: 0.98 % / Rmerge(I) obs: 0.083 |
-Processing
Software |
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Refinement | Resolution: 2.48→6 Å / σ(F): 4 /
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Refinement step | Cycle: LAST / Resolution: 2.48→6 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.19 | ||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |