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- PDB-2al2: Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0 -
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Open data
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Basic information
Entry | Database: PDB / ID: 2al2 | ||||||
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Title | Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0 | ||||||
![]() | (enolase 1) x 2 | ||||||
![]() | LYASE / BETA BARREL | ||||||
Function / homology | ![]() Gluconeogenesis / regulation of vacuole fusion, non-autophagic / Glycolysis / melatonin binding / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / fungal-type vacuole / glycolytic process / magnesium ion binding ...Gluconeogenesis / regulation of vacuole fusion, non-autophagic / Glycolysis / melatonin binding / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / fungal-type vacuole / glycolytic process / magnesium ion binding / mitochondrion / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sims, P.A. / Menefee, A.L. / Larsen, T.M. / Mansoorabadi, S.O. / Reed, G.H. | ||||||
![]() | ![]() Title: Structure and catalytic properties of an engineered heterodimer of enolase composed of one active and one inactive subunit Authors: Sims, P.A. / Menefee, A.L. / Larsen, T.M. / Mansoorabadi, S.O. / Reed, G.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.6 KB | Display | ![]() |
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PDB format | ![]() | 149.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 475.1 KB | Display | ![]() |
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Full document | ![]() | 490.8 KB | Display | |
Data in XML | ![]() | 38.9 KB | Display | |
Data in CIF | ![]() | 56.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 46674.695 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: active form Source: (gene. exp.) ![]() ![]() Gene: ENO1, ENOA, HSP48 / Plasmid: PetEnol / Production host: ![]() ![]() |
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#2: Protein | Mass: 46734.766 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: inactive form Source: (gene. exp.) ![]() ![]() Gene: ENO1, ENOA, HSP48 / Plasmid: PetEnol / Production host: ![]() ![]() |
-Non-polymers , 6 types, 545 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/PEP.gif)
![](data/chem/img/2PG.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PEP.gif)
![](data/chem/img/2PG.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-MG / #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-CL / | #7: Chemical | ChemComp-K / | #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 42.2 % |
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Crystal grow | Temperature: 293 K / Method: batch / pH: 8 Details: PEG 8000, potasssium chloride, pH 8.0, Batch, temperature 293K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: BRUKER / Detector: CCD / Date: Apr 4, 2005 |
Radiation | Monochromator: Pt-135 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→26 Å / Num. all: 67650 / Num. obs: 67650 / % possible obs: 100 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.85→26 Å
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Refine LS restraints |
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