+Open data
-Basic information
Entry | Database: PDB / ID: 2a9x | ||||||
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Title | TAR RNA recognition by a cyclic peptidomimetic of Tat protein | ||||||
Components |
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Keywords | RNA binding protein/RNA / NMR / peptidomimetics / peptide structure / RNA recognition / immunodeficiency virus / TAR RNA / RNA binding protein-RNA COMPLEX | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / Torsion Angle Molecular Dynamics | ||||||
Authors | Leeper, T.C. / Athanassiou, Z. / Dias, R.L. / Robinson, J.A. / Varani, G. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: TAR RNA recognition by a cyclic peptidomimetic of Tat protein. Authors: Leeper, T.C. / Athanassiou, Z. / Dias, R.L. / Robinson, J.A. / Varani, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2a9x.cif.gz | 218.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2a9x.ent.gz | 189.4 KB | Display | PDB format |
PDBx/mmJSON format | 2a9x.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2a9x_validation.pdf.gz | 375.7 KB | Display | wwPDB validaton report |
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Full document | 2a9x_full_validation.pdf.gz | 550.1 KB | Display | |
Data in XML | 2a9x_validation.xml.gz | 34.6 KB | Display | |
Data in CIF | 2a9x_validation.cif.gz | 47.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a9/2a9x ftp://data.pdbj.org/pub/pdb/validation_reports/a9/2a9x | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 8923.310 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was transcribed in vitro from DNA oligonucleotide templates with T7 RNA polymerase purified in house with unlabeled or 13C/15N enriched NTPs (Silantes) and purified by denaturing PAGE. |
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#2: Protein/peptide | Mass: 1754.162 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The BIV-2 peptide was synthesized by solid phase synthesis and purified by reverse phase HPLC |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Torsion Angle Molecular Dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 |