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Open data
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Basic information
Entry | Database: PDB / ID: 2a9x | ||||||
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Title | TAR RNA recognition by a cyclic peptidomimetic of Tat protein | ||||||
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![]() | RNA binding protein/RNA / NMR / peptidomimetics / peptide structure / RNA recognition / immunodeficiency virus / TAR RNA / RNA binding protein-RNA COMPLEX | ||||||
Function / homology | RNA / RNA (> 10)![]() | ||||||
Method | SOLUTION NMR / Torsion Angle Molecular Dynamics | ||||||
![]() | Leeper, T.C. / Athanassiou, Z. / Dias, R.L. / Robinson, J.A. / Varani, G. | ||||||
![]() | ![]() Title: TAR RNA recognition by a cyclic peptidomimetic of Tat protein. Authors: Leeper, T.C. / Athanassiou, Z. / Dias, R.L. / Robinson, J.A. / Varani, G. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 218.1 KB | Display | ![]() |
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PDB format | ![]() | 189.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 375.7 KB | Display | ![]() |
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Full document | ![]() | 550.1 KB | Display | |
Data in XML | ![]() | 34.6 KB | Display | |
Data in CIF | ![]() | 47.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 8923.310 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was transcribed in vitro from DNA oligonucleotide templates with T7 RNA polymerase purified in house with unlabeled or 13C/15N enriched NTPs (Silantes) and purified by denaturing PAGE. |
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#2: Protein/peptide | Mass: 1754.162 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The BIV-2 peptide was synthesized by solid phase synthesis and purified by reverse phase HPLC |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
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Refinement | Method: Torsion Angle Molecular Dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 10 |