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- PDB-2a8e: Three-dimensional structure of Bacillus subtilis Q45498 putative ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2a8e | ||||||
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Title | Three-dimensional structure of Bacillus subtilis Q45498 putative protein at resolution 2.5A. Northeast Structural Genomics Consortium target SR204. | ||||||
![]() | hypothetical protein yktB | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / NESG / Q45498 / yktB / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium | ||||||
Function / homology | Protein of unknown function UPF0637 / Protein of unknown function (DUF1054) / Protein of unknown function DUF1054 / BirA Bifunctional Protein; domain 2 / 2-Layer Sandwich / Alpha Beta / DI(HYDROXYETHYL)ETHER / UPF0637 protein YktB![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kuzin, A.P. / Su, M. / Yong, W. / Vorobiev, S. / Acton, T. / Xiao, R. / Conover, K. / Ma, L.-C. / Cunningham, K.E. / Montelione, G.T. ...Kuzin, A.P. / Su, M. / Yong, W. / Vorobiev, S. / Acton, T. / Xiao, R. / Conover, K. / Ma, L.-C. / Cunningham, K.E. / Montelione, G.T. / Hunt, J.F. / Tong, L. / Northeast Structural Genomics Consortium (NESG) | ||||||
![]() | ![]() Title: Three-dimensional structure of Bacillus subtilis Q45498 putative protein at resolution 2.5A. Northeast Structural Genomics Consortium target SR204. Authors: Kuzin, A.P. / Su, M. / Yong, W. / Vorobiev, S. / Acton, T. / Xiao, R. / Conover, K. / Ma, L.-C. / Cunningham, K.E. / Montelione, G.T. / Hunt, J.F. / Tong, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.3 KB | Display | ![]() |
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PDB format | ![]() | 43.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.8 KB | Display | ![]() |
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Full document | ![]() | 454.8 KB | Display | |
Data in XML | ![]() | 11.3 KB | Display | |
Data in CIF | ![]() | 15.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26002.734 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-PEG / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.4 Å3/Da / Density % sol: 63.5 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 26% PEG 4000, 0.2M Li(2)So(4), 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 16, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97922 Å / Relative weight: 1 |
Reflection | Resolution: 2→30 Å / Num. all: 34753 / % possible obs: 77.4 % / Observed criterion σ(I): -3 / Redundancy: 5.45 % / Biso Wilson estimate: 26.8 Å2 / Rmerge(I) obs: 0.058 |
Reflection shell | Resolution: 2.52→2.71 Å / Rmerge(I) obs: 0.109 / % possible all: 78.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 22.0893 Å2 / ksol: 0.330621 e/Å3 | ||||||||||||||||||||
Displacement parameters | Biso mean: 30.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.5→29.79 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.024 / Total num. of bins used: 6
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Xplor file |
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