MitochondrialimportreceptorsubunitTOM20-3 / Translocase of outer membrane 20 kDa isoform 3
Mass: 17976.834 Da / Num. of mol.: 1 / Fragment: isoform-3 cytosolic receptor domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: TOM20-3 / Plasmid: pGEX-6P-3 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta [BL21(DE3) derived] / References: UniProt: P82874
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D 13C-separated NOESY
1
2
2
3D 15N-separated NOESY
1
3
2
HNHA
1
4
3
3D-13C-separated NOESY (aromatics)
1
5
3
3D 13C-separated NOESY
NMR details
Text: Structure contains 5 non-native residues at the N-terminus (from fusion system protease site) and 8 non-native residues at the C-terminus from a polyhistidine tag.
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
600
1
Varian INOVA
Varian
INOVA
500
2
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Processing
NMR software
Name
Version
Developer
Classification
VNMR
4.1c
VarianInc.
collection
NMRPipe
1.1
Delaglio, F.
processing
Sparky
3.111
Goddard, T.D & Kneller, D.G.
dataanalysis
CYANA
1.0.7
Guentert, P.
structuresolution
CANDID
1
Guentert, P. & Herrmann, T.
dataanalysis
CYANA
1.0.7
Guentert, P.
refinement
Refinement
Method: torsion angle dynamics / Software ordinal: 1 Details: The CANDID software was used for initial automatic NOE assignment. Structures are based on 1629 NOE-derived upper distance constraints (47 intra residue), 136 distance restraints from ...Details: The CANDID software was used for initial automatic NOE assignment. Structures are based on 1629 NOE-derived upper distance constraints (47 intra residue), 136 distance restraints from hydrogen bonds, refinement against 129 J(HNHA) coupling constants and 234 PHI/PSI dihedral angle constraints from TALOS.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20
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