+Open data
-Basic information
Entry | Database: PDB / ID: 1ziq | ||||||
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Title | Deuterated gammaE crystallin in D2O solvent | ||||||
Components | Gamma crystallin E | ||||||
Keywords | STRUCTURAL PROTEIN / 4 Greek key motifs | ||||||
Function / homology | Function and homology information structural constituent of eye lens / lens development in camera-type eye / visual perception Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.72 Å | ||||||
Authors | Artero, J.B. / Hartlein, M. / McSweeney, S. / Timmins, P. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2005 Title: A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O. Authors: Artero, J.B. / Hartlein, M. / McSweeney, S. / Timmins, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ziq.cif.gz | 55.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ziq.ent.gz | 39.9 KB | Display | PDB format |
PDBx/mmJSON format | 1ziq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zi/1ziq ftp://data.pdbj.org/pub/pdb/validation_reports/zi/1ziq | HTTPS FTP |
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-Related structure data
Related structure data | 1zgtC 1zieC 1zirC 1a5dS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21163.611 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Plasmid: pET28a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P02528 | ||
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#2: Chemical | #3: Chemical | ChemComp-DOD / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 36.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: PEG 3350, Magnesium Acetate, D2O, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.9393 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 11, 2004 |
Radiation | Monochromator: A double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9393 Å / Relative weight: 1 |
Reflection | Resolution: 1.72→50.63 Å / Num. all: 18476 / Num. obs: 18328 / % possible obs: 99.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 1.72→1.85 Å / % possible all: 89.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1A5D Resolution: 1.72→50.63 Å / σ(F): 4 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.72→50.63 Å
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Refine LS restraints |
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