- PDB-1zfs: Solution structure of S100A1 bound to calcium -
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基本情報
登録情報
データベース: PDB / ID: 1zfs
タイトル
Solution structure of S100A1 bound to calcium
要素
S-100 protein, alpha chain
キーワード
METAL BINDING PROTEIN / S100 / noncovalent homodimer / x-type 4 helix bundle / calcium binding / conformational change
機能・相同性
機能・相同性情報
Regulation of TLR by endogenous ligand / RAGE receptor binding / M band / I band / S100 protein binding / A band / regulation of heart contraction / positive regulation of sprouting angiogenesis / sarcoplasmic reticulum / Z disc ...Regulation of TLR by endogenous ligand / RAGE receptor binding / M band / I band / S100 protein binding / A band / regulation of heart contraction / positive regulation of sprouting angiogenesis / sarcoplasmic reticulum / Z disc / calcium-dependent protein binding / ATPase binding / positive regulation of canonical NF-kappaB signal transduction / calcium ion binding / positive regulation of cell population proliferation / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / protein homodimerization activity / mitochondrion / extracellular space / identical protein binding / nucleus / cytoplasm 類似検索 - 分子機能
Protein S100-A1 / S-100/ICaBP type calcium binding protein signature. / S100/Calcium binding protein 7/8-like, conserved site / S100/CaBP-9k-type, calcium binding, subdomain / S-100/ICaBP type calcium binding domain / S-100/ICaBP type calcium binding domain / EF hand / EF-hand / Recoverin; domain 1 / EF-hand, calcium binding motif ...Protein S100-A1 / S-100/ICaBP type calcium binding protein signature. / S100/Calcium binding protein 7/8-like, conserved site / S100/CaBP-9k-type, calcium binding, subdomain / S-100/ICaBP type calcium binding domain / S-100/ICaBP type calcium binding domain / EF hand / EF-hand / Recoverin; domain 1 / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
Text: RDC data using radially and axially compressed acrylamide gels were added into structure calculations. H-bonds were determined using hydrogen exchange experiments
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試料調製
詳細
内容: 0.5-3 mM S100A1 monomer, U-15N, 13C, 0.35 mM NaN3, 15 mM NaCl, 20 mM CaCl2, 25 mM Tris, 25 mM DTT, 10% D2O 溶媒系: 90% H2O/10% D2O
試料状態
イオン強度: 15 mM / pH: 7.2 / 圧: ambient / 温度: 310 K
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
600
1
Bruker AVANCE
Bruker
AVANCE
800
2
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解析
NMR software
名称
バージョン
開発者
分類
X-PLOR
2.9.3
C.D. Schwieters, J.J. Kuszewski, N. Tjandra, G.M. Clore
構造決定
X-PLOR
2.9.3
C.D. Schwieters, J.J. Kuszewski, N. Tjandra, G.M. Clore