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Open data
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Basic information
Entry | Database: PDB / ID: 1yc7 | ||||||
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Title | cAbAn33 VHH fragment against VSG | ||||||
![]() | anti-VSG immunoglobulin heavy chain variable domain cAbAn33 | ||||||
![]() | IMMUNE SYSTEM / antibody / camel antibody | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Conrath, K. / Vincke, C. / Stijlemans, B. / Schymkowitz, J. / Wyns, L. / Muyldermans, S. / Loris, R. | ||||||
![]() | ![]() Title: Antigen Binding and Solubility Effects upon the Veneering of a Camel VHH in Framework-2 to Mimic a VH. Authors: Conrath, K. / Vincke, C. / Stijlemans, B. / Schymkowitz, J. / Decanniere, K. / Wyns, L. / Muyldermans, S. / Loris, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58 KB | Display | ![]() |
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PDB format | ![]() | 45.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 13795.182 Da / Num. of mol.: 2 / Fragment: cAbAn33 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.79 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG-4000, ammomium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: May 27, 2001 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.81 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20 Å / Num. all: 31676 / Num. obs: 31676 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.9 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 17.3 |
Reflection shell | Resolution: 1.6→1.66 Å / Redundancy: 1.69 % / Rmerge(I) obs: 0.226 / Mean I/σ(I) obs: 3.1 / Num. unique all: 2802 / % possible all: 86.9 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.6→20 Å
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Refine LS restraints |
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