+Open data
-Basic information
Entry | Database: PDB / ID: 1xw2 | ||||||
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Title | Structure Of A Cold-Adapted Family 8 Xylanase | ||||||
Components | Endo-1,4-beta-Xylanase | ||||||
Keywords | HYDROLASE / XYLAN DEGRADATION / PSYCHROPHILIC / COLD ADAPTATION / TEMPERATURE / GLYCOSYL HYDROLASE / FAMILY 8 | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pseudoalteromonas haloplanktis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.76 Å | ||||||
Authors | Collins, T. / De Vos, D. / Hoyoux, A. / Savvides, S.N. / Gerday, C. / Van Beeumen, J. / Feller, G. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2005 Title: Study of the active site residues of a glycoside hydrolase family 8 xylanase Authors: Collins, T. / De Vos, D. / Hoyoux, A. / Savvides, S.N. / Gerday, C. / Van Beeumen, J. / Feller, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1xw2.cif.gz | 99.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1xw2.ent.gz | 78.8 KB | Display | PDB format |
PDBx/mmJSON format | 1xw2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1xw2_validation.pdf.gz | 414.7 KB | Display | wwPDB validaton report |
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Full document | 1xw2_full_validation.pdf.gz | 415.4 KB | Display | |
Data in XML | 1xw2_validation.xml.gz | 20.5 KB | Display | |
Data in CIF | 1xw2_validation.cif.gz | 32.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xw/1xw2 ftp://data.pdbj.org/pub/pdb/validation_reports/xw/1xw2 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46023.785 Da / Num. of mol.: 1 / Mutation: E78Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudoalteromonas haloplanktis (bacteria) Plasmid: pET22b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q8RJN8, endo-1,4-beta-xylanase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: MPD, sodium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR591 / Wavelength: 1.5418 |
Detector | Type: MAC Science DIP-2030 / Detector: IMAGE PLATE / Date: Oct 1, 2003 |
Radiation | Monochromator: Montel Multilayer Optics / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→50 Å / Num. all: 46299 / Num. obs: 46033 / % possible obs: 99.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 1.76→1.79 Å / % possible all: 95.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.76→50 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.95 / SU B: 1.607 / SU ML: 0.053 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.105 / ESU R Free: 0.094 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.578 Å2
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Refinement step | Cycle: LAST / Resolution: 1.76→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.76→1.806 Å / Total num. of bins used: 20 /
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