ジャーナル: Nucleic Acids Res. / 年: 2004 タイトル: Change of RNase P RNA function by single base mutation correlates with perturbation of metal ion binding in P4 as determined by NMR spectroscopy 著者: Schmitz, M.
Text: The structure was determined using standard 2D homonuclear techniques as well as 13C and 31P heteronuclear experiments performed at natural abundance. Intermolecular NOE crosspeaks between RNA ...Text: The structure was determined using standard 2D homonuclear techniques as well as 13C and 31P heteronuclear experiments performed at natural abundance. Intermolecular NOE crosspeaks between RNA protons and cobalt (III) hexammine protons and intermolecular distance constraints derived thereof were used to determine the site of cobalt (III) hexammine binding.
タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz
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解析
NMR software
名称
バージョン
開発者
分類
XwinNMR
1.2
Bruker
collection
NMRPipe
2.1
F. Delaglio
解析
X-PLOR
3.1
A. Bruenger
構造決定
X-PLOR
3.1
A. Bruenger
精密化
精密化
手法: restrained molecular dynamics, simulated annealing / ソフトェア番号: 1 詳細: The average structure is based on the superposition of 18 structures after refinement. The average RMS deviation between the ensemble and the average structure is 1.87 Angstrom. A total of ...詳細: The average structure is based on the superposition of 18 structures after refinement. The average RMS deviation between the ensemble and the average structure is 1.87 Angstrom. A total of 261 NOE-derived distance constraints, 246 dihedral constraints and 48 distance constraints from hydrogen bonds were used in refinement. 12 NOE derived intermolecular distance constraints were used to localize the bound cobalt (III) hexammine.