ジャーナル: Nucleic Acids Res. / 年: 2004 タイトル: Change of RNase P RNA function by single base mutation correlates with perturbation of metal ion binding in P4 as determined by NMR spectroscopy 著者: Schmitz, M.
分子量: 8633.143 Da / 分子数: 1 / 由来タイプ: 合成 詳細: enzymatically synthesized from DNA oligonucleotide template by T7 RNA polymerase
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY
1
2
2
2D NOESY
1
3
2
DQF-COSY
1
4
2
31P-1H-HETERO-COSY
1
5
2
31P-1H-HETERO-TOCSY-NOESY
NMR実験の詳細
Text: The structure was determined using standard 2D homonuclear techniques as well as 13C and 31P heteronuclear experiments performed at natural abundance
タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz
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解析
NMR software
名称
バージョン
開発者
分類
XwinNMR
1.2
Bruker
collection
NMRPipe
2.1
F. Delaglio
解析
X-PLOR
3.1
構造決定
X-PLOR
3.1
精密化
精密化
手法: restrained molecular dynamics, simulated annealing / ソフトェア番号: 1 詳細: The average structure is based on the superposition of 14 structures after refinement. The average RMS deviation between the ensemble and the average structure is 1.77 Angstrom. A total of ...詳細: The average structure is based on the superposition of 14 structures after refinement. The average RMS deviation between the ensemble and the average structure is 1.77 Angstrom. A total of 261 NOE-derived distance constraints, 246 dihedral restraints and 48 distance restraints from hydrogen bonds were used in refinement.