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- PDB-1xqy: Crystal structure of F1-mutant S105A complex with PRO-LEU-GLY-GLY -
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Open data
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Basic information
Entry | Database: PDB / ID: 1xqy | ||||||
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Title | Crystal structure of F1-mutant S105A complex with PRO-LEU-GLY-GLY | ||||||
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![]() | HYDROLASE / ALPHA-BETA HYDROLASE / CAGED ACTIVE SITE / CATALYTIC TRIAD / NUCLEOPHILE / PEPTIDE CLEAVAGE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Goettig, P. / Brandstetter, H. / Groll, M. / Goehring, W. / Konarev, P.V. / Svergun, D.I. / Huber, R. / Kim, J.-S. | ||||||
![]() | ![]() Title: X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum Authors: Goettig, P. / Brandstetter, H. / Groll, M. / Goehring, W. / Konarev, P.V. / Svergun, D.I. / Huber, R. / Kim, J.-S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.2 KB | Display | ![]() |
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PDB format | ![]() | 54.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.9 KB | Display | ![]() |
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Full document | ![]() | 469.9 KB | Display | |
Data in XML | ![]() | 15.6 KB | Display | |
Data in CIF | ![]() | 20.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1xqvC ![]() 1xqwC ![]() 1xqxC ![]() 1xrlC ![]() 1xrmC ![]() 1xrnC ![]() 1xroC ![]() 1xrpC ![]() 1xrqC ![]() 1xrrC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 33514.090 Da / Num. of mol.: 1 / Mutation: S105A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 342.391 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: chemically synthesized |
#3: Chemical | ChemComp-PRO / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.18 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG 6000, Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 16, 2003 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→19.7 Å / Num. all: 5742 / Num. obs: 5642 / % possible obs: 98.3 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Net I/σ(I): 10.1 |
Reflection shell | Resolution: 2.83→3 Å / % possible all: 55.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 59.8606 Å2 / ksol: 0.26791 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.2→19.66 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.2→3.4 Å / Rfactor Rfree error: 0.076 / Total num. of bins used: 6
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Xplor file |
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