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- PDB-1xg2: Crystal structure of the complex between pectin methylesterase an... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1xg2 | ||||||
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Title | Crystal structure of the complex between pectin methylesterase and its inhibitor protein | ||||||
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![]() | HYDROLASE/HYDROLASE INHIBITOR / PROTEIN-PROTEIN COMPLEX / BETA HELIX / FOUR HELIX BUNDLE / HYDROLASE-HYDROLASE INHIBITOR COMPLEX | ||||||
Function / homology | ![]() pectinesterase inhibitor activity / fruit ripening / pectinesterase / pectinesterase activity / : / cell wall modification / pectin catabolic process / cell wall / extracellular region / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Di Matteo, A. / Raiola, A. / Camardella, L. / Giovane, A. / Bonivento, D. / De Lorenzo, G. / Cervone, F. / Bellincampi, D. / Tsernoglou, D. | ||||||
![]() | ![]() Title: Structural Basis for the Interaction between Pectin Methylesterase and a Specific Inhibitor Protein Authors: Di Matteo, A. / Giovane, A. / Raiola, A. / Camardella, L. / Bonivento, D. / De Lorenzo, G. / Cervone, F. / Bellincampi, D. / Tsernoglou, D. #1: Journal: Biochim.Biophys.Acta / Year: 2004 Title: Pectin methylesterase inhibitor Authors: Giovane, A. / Servillo, L. / Balestrieri, C. / Raiola, A. / D'Avino, R. / Tamburrini, M. / Ciardiello, M.A. / Camardella, L. #2: Journal: Proteins / Year: 2003 Title: Tomato pectin methylesterase: modeling, fluorescence, and inhibitor interaction studies-comparison with the bacterial (Erwinia chrysanthemi) enzyme Authors: D'Avino, R. / Camardella, L. / Christensen, T.M. / Giovane, A. / Servillo, L. #3: Journal: Trends Plant Sci. / Year: 2001 Title: Pectin methylesterases: cell wall enzymes with important roles in plant physiology Authors: Micheli, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 107.8 KB | Display | ![]() |
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PDB format | ![]() | 86.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34586.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Protein | Mass: 16438.283 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.49 Å3/Da / Density % sol: 64.46 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 6 Details: magnesium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 18, 2004 / Details: mirrors |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. all: 56180 / Num. obs: 56180 / % possible obs: 97.5 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.082 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.338 / % possible all: 92.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.18 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.901→1.95 Å / Total num. of bins used: 20 /
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