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Yorodumi- PDB-1xdt: COMPLEX OF DIPHTHERIA TOXIN AND HEPARIN-BINDING EPIDERMAL GROWTH ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1xdt | ||||||
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Title | COMPLEX OF DIPHTHERIA TOXIN AND HEPARIN-BINDING EPIDERMAL GROWTH FACTOR | ||||||
Components |
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Keywords | COMPLEX (TOXIN/GROWTH FACTOR) / COMPLEX (TOXIN-GROWTH FACTOR) / DIPHTHERIA TOXIN / RECEPTOR / HEPARIN-BINDING EPIDERMAL GROWTH FACTOR / EPIDERMAL GROWTH FACTOR / COMPLEX (TOXIN-GROWTH FACTOR) complex | ||||||
Function / homology | Function and homology information negative regulation of elastin biosynthetic process / NAD+-diphthamide ADP-ribosyltransferase / NAD+-diphthamide ADP-ribosyltransferase activity / ERBB2-ERBB4 signaling pathway / positive regulation of keratinocyte migration / PI3K events in ERBB4 signaling / wound healing, spreading of epidermal cells / Inhibition of Signaling by Overexpressed EGFR / regulation of heart contraction / EGFR interacts with phospholipase C-gamma ...negative regulation of elastin biosynthetic process / NAD+-diphthamide ADP-ribosyltransferase / NAD+-diphthamide ADP-ribosyltransferase activity / ERBB2-ERBB4 signaling pathway / positive regulation of keratinocyte migration / PI3K events in ERBB4 signaling / wound healing, spreading of epidermal cells / Inhibition of Signaling by Overexpressed EGFR / regulation of heart contraction / EGFR interacts with phospholipase C-gamma / muscle organ development / ERBB2-EGFR signaling pathway / PTK6 promotes HIF1A stabilization / epidermal growth factor receptor binding / ERBB2 Activates PTK6 Signaling / Uptake and function of diphtheria toxin / positive regulation of wound healing / Signaling by EGFR / transmembrane receptor protein tyrosine kinase activator activity / Signaling by ERBB4 / ERBB2 Regulates Cell Motility / PI3K events in ERBB2 signaling / protein transmembrane transporter activity / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / SHC1 events in ERBB4 signaling / GAB1 signalosome / Nuclear signaling by ERBB4 / Signaling by ERBB2 / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / EGFR Transactivation by Gastrin / GRB2 events in ERBB2 signaling / nucleotidyltransferase activity / SHC1 events in ERBB2 signaling / cell chemotaxis / EGFR downregulation / positive regulation of smooth muscle cell proliferation / Signaling by ERBB2 TMD/JMD mutants / growth factor activity / clathrin-coated endocytic vesicle membrane / epidermal growth factor receptor signaling pathway / Signaling by ERBB2 KD Mutants / Downregulation of ERBB2 signaling / Constitutive Signaling by Aberrant PI3K in Cancer / endocytic vesicle membrane / positive regulation of peptidyl-tyrosine phosphorylation / Cargo recognition for clathrin-mediated endocytosis / PIP3 activates AKT signaling / Clathrin-mediated endocytosis / heparin binding / toxin activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / positive regulation of cell growth / Extra-nuclear estrogen signaling / receptor ligand activity / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / positive regulation of cell population proliferation / cell surface / signal transduction / extracellular space / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Corynebacterium diphtheriae (bacteria) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.65 Å | ||||||
Authors | Louie, G.V. / Yang, W. / Bowman, M.E. / Choe, S. | ||||||
Citation | Journal: Mol.Cell / Year: 1997 Title: Crystal structure of the complex of diphtheria toxin with an extracellular fragment of its receptor. Authors: Louie, G.V. / Yang, W. / Bowman, M.E. / Choe, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1xdt.cif.gz | 116.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1xdt.ent.gz | 93.1 KB | Display | PDB format |
PDBx/mmJSON format | 1xdt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xd/1xdt ftp://data.pdbj.org/pub/pdb/validation_reports/xd/1xdt | HTTPS FTP |
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-Related structure data
Related structure data | 1mdtS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | DIPHTHERIA TOXIN MOLECULE HAS THE OPEN CONFORMATION, AND DIMERIZES WITH ANOTHER DIPHTHERIA TOXIN MOLECULE. |
-Components
#1: Protein | Mass: 58411.359 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Corynebacterium diphtheriae (bacteria) / Cellular location: EXTRACELLULARGlossary of biology / Variant: LYSOGENIZED BY THE CORYNEPHAGE BETA / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 References: UniProt: P00588, NAD+-diphthamide ADP-ribosyltransferase |
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#2: Protein | Mass: 8927.515 Da / Num. of mol.: 1 / Fragment: EXTRACELLULAR DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Cellular location: MEMBRANE-ANCHORED OR EXTRACELLULARLY-RELEASED Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q99075 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion / pH: 7.5 Details: PROTEIN (15 MG/ML) IN 10 MM TRIS-HCL (PH 7.5), 0.15 M NACL MIXED WITH AN EQUAL VOLUME OF PRECIPITANT CONTAINING 50 MM TRIS-HCL (PH 7.5), 0.15 M NACL, 5% (V/V) GLYCEROL, 22-30% (W/V) ...Details: PROTEIN (15 MG/ML) IN 10 MM TRIS-HCL (PH 7.5), 0.15 M NACL MIXED WITH AN EQUAL VOLUME OF PRECIPITANT CONTAINING 50 MM TRIS-HCL (PH 7.5), 0.15 M NACL, 5% (V/V) GLYCEROL, 22-30% (W/V) POLYETHYLENE GLYCOL 3350; AND EQUILIBRATED BY VAPOR DIFFUSION AGAINST PRECIPITANT, vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 23 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Oct 1, 1996 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.65→25 Å / Num. obs: 54532 / % possible obs: 88.5 % / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Rmerge(I) obs: 0.052 / Rsym value: 0.052 / Net I/σ(I): 27.9 |
Reflection shell | Resolution: 2.65→2.74 Å / Redundancy: 2.1 % / Rmerge(I) obs: 0.152 / Mean I/σ(I) obs: 5 / Rsym value: 0.152 / % possible all: 79.2 |
Reflection shell | *PLUS % possible obs: 79.2 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1MDT Resolution: 2.65→25 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 37.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.65→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.65→2.77 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.851 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.29 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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