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- PDB-1xat: STRUCTURE OF THE HEXAPEPTIDE XENOBIOTIC ACETYLTRANSFERASE FROM PS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1xat | ||||||
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Title | STRUCTURE OF THE HEXAPEPTIDE XENOBIOTIC ACETYLTRANSFERASE FROM PSEUDOMONAS AERUGINOSA | ||||||
![]() | XENOBIOTIC ACETYLTRANSFERASE | ||||||
![]() | ACETYLTRANSFERASE / XENOBIOTIC / CHLORAMPHENICOL / LEFT-HANDED BETA HELIX | ||||||
Function / homology | ![]() chloramphenicol O-acetyltransferase activity / chloramphenicol O-acetyltransferase / response to antibiotic Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Beaman, T.W. / Sugantino, M. / Roderick, S.L. | ||||||
![]() | ![]() Title: Structure of the hexapeptide xenobiotic acetyltransferase from Pseudomonas aeruginosa. Authors: Beaman, T.W. / Sugantino, M. / Roderick, S.L. #1: ![]() Title: Purification and Crystallization of Pseudomonas Aeruginosa Chloramphenicol Acetyltransferase Authors: Tian, Y. / Beaman, T.W. / Roderick, S.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 45.9 KB | Display | ![]() |
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PDB format | ![]() | 33.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 360.7 KB | Display | ![]() |
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Full document | ![]() | 366.1 KB | Display | |
Data in XML | ![]() | 5.9 KB | Display | |
Data in CIF | ![]() | 8.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23506.443 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 6.6 Å3/Da / Density % sol: 79 % | ||||||||||||||||||||||||
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Crystal grow | pH: 8.5 Details: 10-20% POLYETHYLENEGLYCOL MONOMETHYL ETHER 2000, 100 MM TRIS, PH 8.5, 10 MM NICL2 | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Aug 1, 1997 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→99 Å / Num. obs: 10448 / % possible obs: 95.1 % / Redundancy: 3.2 % / Biso Wilson estimate: 24.8 Å2 / Rmerge(I) obs: 0.078 / Rsym value: 0.078 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 3.2→3.3 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.165 / Mean I/σ(I) obs: 8 / Rsym value: 0.165 / % possible all: 91.1 |
Reflection | *PLUS Num. measured all: 34784 |
Reflection shell | *PLUS % possible obs: 91.1 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 21.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.2→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.2→3.39 Å / Rfactor Rfree error: 0.034 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.264 |