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Yorodumi- PDB-1x3u: Solution structure of the C-terminal transcriptional activator do... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1x3u | ||||||
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Title | Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot | ||||||
Components | Transcriptional regulatory protein fixJ | ||||||
Keywords | TRANSCRIPTION / HELIX-TURN-HELIX | ||||||
Function / homology | Function and homology information nitrogen fixation / DNA-binding transcription activator activity / phosphorelay signal transduction system / protein-DNA complex / transcription cis-regulatory region binding / positive regulation of DNA-templated transcription / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Sinorhizobium meliloti (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Kurashima-Ito, K. / Kasai, Y. / Hosono, K. / Tamura, K. / Oue, S. / Isogai, M. / Ito, Y. / Nakamura, H. / Shiro, Y. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium meliloti and its recognition of the fixK promoter Authors: Kurashima-Ito, K. / Kasai, Y. / Hosono, K. / Tamura, K. / Oue, S. / Isogai, M. / Ito, Y. / Nakamura, H. / Shiro, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1x3u.cif.gz | 469.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1x3u.ent.gz | 408 KB | Display | PDB format |
PDBx/mmJSON format | 1x3u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x3/1x3u ftp://data.pdbj.org/pub/pdb/validation_reports/x3/1x3u | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8580.907 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sinorhizobium meliloti (bacteria) / Gene: fixJ / Production host: Escherichia coli (E. coli) / References: UniProt: P10958 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1mM FixJC U-15N,13C; 20mM NaHPO4-NaH2PO4 (pH 5.5), 100mM NaCl, 50mM Na2SO4; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 5.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 80 / Conformers submitted total number: 20 |