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- PDB-1wrt: NMR STUDY OF APO TRP REPRESSOR -

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Basic information

Entry
Database: PDB / ID: 1wrt
TitleNMR STUDY OF APO TRP REPRESSOR
ComponentsAPO TRP REPRESSOR
KeywordsOPERON REPRESSOR / TRANSCRIPTION REGULATION / DNA-BINDING
Function / homology
Function and homology information


sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / DNA binding / cytoplasm
Similarity search - Function
TrpR-like / Trp repressor, bacterial / Trp repressor / TrpR-like superfamily / Trp repressor protein / Trp repressor/replication initiator / Trp Operon Repressor; Chain A / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Trp operon repressor
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodSOLUTION NMR
AuthorsZhao, D. / Zheng, Z.
Citation
Journal: J.Mol.Biol. / Year: 1993
Title: Refined solution structures of the Escherichia coli trp holo- and aporepressor.
Authors: Zhao, D. / Arrowsmith, C.H. / Jia, X. / Jardetzky, O.
#1: Journal: J.Mol.Biol. / Year: 1994
Title: The Solution Structures of the Trp Repressor-Operator DNA Complex
Authors: Zhang, H. / Zhao, D. / Revington, M. / Lee, W. / Jia, X. / Arrowsmith, C. / Jardetzky, O.
#2: Journal: J.Phys.Chem. / Year: 1993
Title: Sequential Simulated Annealing: An Efficient Procedure for Structural Refinement Based on NMR Constraints
Authors: Zhao, D. / Jardetzky, O.
#3: Journal: Eur.J.Biochem. / Year: 1991
Title: The Solution Structures of Escherichia Coli Trp Repressor and Trp Aporepressor at an Intermediate Resolution
Authors: Arrowsmith, C. / Pachter, R. / Altman, R. / Jardetzky, O.
#4: Journal: Biochemistry / Year: 1990
Title: Sequence-Specific 1H NMR Assignments and Secondary Structure in Solution of Escherichia Coli Trp Repressor
Authors: Arrowsmith, C.H. / Pachter, R. / Altman, R.B. / Iyer, S.B. / Jardetzky, O.
#5: Journal: Biochemistry / Year: 1989
Title: NMR Assignments for the Amino-Terminal Residues of Trp Repressor and Their Role in DNA Binding
Authors: Arrowsmith, C.H. / Carey, J. / Treat-Clemons, L. / Jardetzky, O.
History
DepositionMay 12, 1995Processing site: BNL
Revision 1.0Jun 20, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
R: APO TRP REPRESSOR
S: APO TRP REPRESSOR


Theoretical massNumber of molelcules
Total (without water)24,0792
Polymers24,0792
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)15 / -
Representative

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Components

#1: Protein APO TRP REPRESSOR


Mass: 12039.727 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / Strain: CY15070 AND CY17071 / References: UniProt: P0A881

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR softwareName: X-PLOR / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 15

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