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Yorodumi- PDB-1wfr: Solution structure of the conserved hypothetical protein TT1886, ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wfr | ||||||
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Title | Solution structure of the conserved hypothetical protein TT1886, possibly sterol carrier protein, from Thermus Thermophilus HB8 | ||||||
Components | Hypothetical Protein TT1886 | ||||||
Keywords | LIPID BINDING PROTEIN / STEROL CARRIER PROTEIN / STRUCTURAL GENOMICS / LIPID BINDING / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | SCP2 sterol-binding domain / SCP-2 sterol transfer family / SCP2 sterol-binding domain / Nonspecific Lipid-transfer Protein; Chain A / SCP2 sterol-binding domain superfamily / 2-Layer Sandwich / Alpha Beta / SCP2 domain-containing protein Function and homology information | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Goroncy, A. / Kigawa, T. / Koshiba, S. / Tomizawa, T. / Kobayashi, N. / Tochio, N. / Inoue, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the conserved hypothetical protein TT1886, possibly sterol carrier protein, from Thermus Thermophilus HB8 Authors: Goroncy, A. / Kigawa, T. / Koshiba, S. / Tomizawa, T. / Kobayashi, N. / Tochio, N. / Inoue, M. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wfr.cif.gz | 931.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wfr.ent.gz | 816.5 KB | Display | PDB format |
PDBx/mmJSON format | 1wfr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1wfr_validation.pdf.gz | 341.1 KB | Display | wwPDB validaton report |
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Full document | 1wfr_full_validation.pdf.gz | 487 KB | Display | |
Data in XML | 1wfr_validation.xml.gz | 58.3 KB | Display | |
Data in CIF | 1wfr_validation.cif.gz | 73 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wf/1wfr ftp://data.pdbj.org/pub/pdb/validation_reports/wf/1wfr | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15078.047 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermus thermophilus (bacteria) / Strain: HB8 / Description: Cell-free protein synthesis / Plasmid: P030826-87 / References: UniProt: Q5SL92 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.09mM TT1886 U-15N, 13C; 20mM phosphate buffer Na; 100mM NaCl, 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 6.0 / Pressure: ambient / Temperature: 318 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest target function | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |