THE FIRST 20 RESIDUES OF Q6IAS6 ARE NOT PART OF THE MATURE ENZYME. THE MATURE PROTEIN COMPRISES ...THE FIRST 20 RESIDUES OF Q6IAS6 ARE NOT PART OF THE MATURE ENZYME. THE MATURE PROTEIN COMPRISES RESIDUES 21-325 AS NUMBERED IN Q6IAS6. A SIX AMINO ACID CLONING ARTIFACT IS ATTACHED TO THE N-TERMINUS OF THE MATURE PROTEIN.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.91 Å3/Da / 溶媒含有率: 57.7 %
結晶化
温度: 277 K 詳細: THE PROTEIN (10 MG/ML) IN 50 MM MES AT PH 6, WAS PASSED THROUGH AN ULTRAFREE-MC 0.22 MM SPIN FILTER UNIT (MILLIPORE) AND ALLOWED TO STAND AT 4 DEGREES C. PURPLE CRYSTALS GREW SPONTANEOUSLY ...詳細: THE PROTEIN (10 MG/ML) IN 50 MM MES AT PH 6, WAS PASSED THROUGH AN ULTRAFREE-MC 0.22 MM SPIN FILTER UNIT (MILLIPORE) AND ALLOWED TO STAND AT 4 DEGREES C. PURPLE CRYSTALS GREW SPONTANEOUSLY OVERNIGHT AND REACHED SIZES UP TO 0.5 MM IN SEVERAL WEEKS.
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データ収集
回折
平均測定温度: 300 K
放射光源
由来: 回転陽極 / タイプ: RIGAKU RU200 / 波長: 1.5418
検出器
タイプ: MAR scanner 345 mm plate / 検出器: IMAGE PLATE / 日付: 2003年8月20日 / 詳細: OSMIC MIRRORS
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1.5418 Å / 相対比: 1
反射
解像度: 2.22→18.98 Å / Num. obs: 15951 / % possible obs: 94.5 % / Observed criterion σ(I): -3 / 冗長度: 4.2 % / Biso Wilson estimate: 36 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 17.97
反射 シェル
解像度: 2.22→2.3 Å / 冗長度: 4.2 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 5.49 / % possible all: 94