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Yorodumi- PDB-1w7i: Crystal Structure Of Myosin V Motor Without nucleotide soaked in ... -
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-Basic information
Entry | Database: PDB / ID: 1w7i | ||||||
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Title | Crystal Structure Of Myosin V Motor Without nucleotide soaked in 10 mM MgADP | ||||||
Components |
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Keywords | MOTOR PROTEIN / UNCONVENTIONAL MYOSIN / MYOSIN V / CHICKEN / MOLECULAR MOTOR / ATPASE / ELC / IQ MOTIF / MUSCLE PROTEIN / MGADP | ||||||
Function / homology | Function and homology information minus-end directed microfilament motor activity / unconventional myosin complex / insulin-responsive compartment / muscle myosin complex / muscle filament sliding / myosin II complex / vesicle transport along actin filament / myosin complex / structural constituent of muscle / microfilament motor activity ...minus-end directed microfilament motor activity / unconventional myosin complex / insulin-responsive compartment / muscle myosin complex / muscle filament sliding / myosin II complex / vesicle transport along actin filament / myosin complex / structural constituent of muscle / microfilament motor activity / filamentous actin / cytoskeletal motor activity / Smooth Muscle Contraction / skeletal muscle tissue development / vesicle-mediated transport / muscle contraction / actin filament organization / protein localization to plasma membrane / cellular response to insulin stimulus / actin filament binding / actin cytoskeleton / vesicle / calmodulin binding / Golgi membrane / calcium ion binding / extracellular exosome / ATP binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | GALLUS GALLUS (chicken) HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Coureux, P.-D. / Sweeney, H.L. / Houdusse, A. | ||||||
Citation | Journal: Embo J. / Year: 2004 Title: Three Myosin V Structures Delineate Essential Features of Chemo-Mechanical Transduction Authors: Coureux, P.-D. / Sweeney, H.L. / Houdusse, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1w7i.cif.gz | 187.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1w7i.ent.gz | 144.5 KB | Display | PDB format |
PDBx/mmJSON format | 1w7i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w7/1w7i ftp://data.pdbj.org/pub/pdb/validation_reports/w7/1w7i | HTTPS FTP |
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-Related structure data
Related structure data | 1w7jC 1w8jC 1oe9S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 91607.172 Da / Num. of mol.: 1 / Fragment: MOTOR DOMAIN, RESIDUES 1-792 Source method: isolated from a genetically manipulated source Details: SOAKED MGADP / Source: (gene. exp.) GALLUS GALLUS (chicken) / Cell line (production host): SF9 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: Q02440 |
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#2: Protein | Mass: 17090.277 Da / Num. of mol.: 1 / Fragment: RESIDUES 59-208 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Cell line (production host): SF9 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: P14649 |
#3: Chemical | ChemComp-ADP / |
#4: Water | ChemComp-HOH / |
Compound details | MYOSIN VA: PROCESSIVE ACTIN-BASED MOTOR THAT CAN MOVE IN LARGE STEPS. POSSIBLY INVOLVED IN ...MYOSIN VA: PROCESSIVE |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 56.7 % |
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Crystal grow | pH: 6.3 Details: 6% PEG 8000, 50 MM MOPS PH 6.5, 2 MM DTT AND 2 MM NAN3 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-1 / Wavelength: 0.934 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 15, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 3→111.8 Å / Num. obs: 21972 / % possible obs: 97.3 % / Redundancy: 6.4 % / Rmerge(I) obs: 0.081 / Net I/σ(I): 18.1 |
Reflection shell | Resolution: 3→3.07 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.284 / Mean I/σ(I) obs: 3.1 / % possible all: 88.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1OE9 Resolution: 3→111.8 Å / SU B: 21.824 / SU ML: 0.411 / ESU R Free: 0.55
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Displacement parameters | Biso mean: 56.7 Å2
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Refinement step | Cycle: LAST / Resolution: 3→111.8 Å
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