+Open data
-Basic information
Entry | Database: PDB / ID: 1w6k | ||||||
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Title | Structure of human OSC in complex with Lanosterol | ||||||
Components | LANOSTEROL SYNTHASE | ||||||
Keywords | ISOMERASE / CYCLASE / CHOLESTEROL / LANOSTEROL / MONOTOPIC MEMBRANE PROTEIN / B-OCTYL-GLUCOSIDE / STEROID BIOSYNTHESIS | ||||||
Function / homology | Function and homology information OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #20 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Prenyltransferase and squalene oxidase repeat / Glycosyltransferase - #20 / Other non-globular / Glycosyltransferase / Alpha/alpha barrel / Special / Mainly Alpha Similarity search - Domain/homology | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.1 Å | ||||||
Authors | Thoma, R. / Schulz-Gasch, T. / D'Arcy, B. / Benz, J. / Aebi, J. / Dehmlow, H. / Hennig, M. / Ruf, A. | ||||||
Citation | Journal: Nature / Year: 2004 Title: Insight Into Steroid Scaffold Formation from the Structure of Human Oxidosqualene Cyclase Authors: Thoma, R. / Schulz-Gasch, T. / D'Arcy, B. / Benz, J. / Aebi, J. / Dehmlow, H. / Hennig, M. / Stihle, M. / Ruf, A. #1: Journal: Biochem.Biophys.Res.Commun. / Year: 2004 Title: The Monotopic Membrane Protein Human Osc is Active as Monomer Authors: Ruf, A. / Muller, F. / D'Arcy, B. / Stihle, M. / Kuznir, E. / Handschin, C. / Morand, O. / Thoma, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1w6k.cif.gz | 180.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1w6k.ent.gz | 143 KB | Display | PDB format |
PDBx/mmJSON format | 1w6k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1w6k_validation.pdf.gz | 567.5 KB | Display | wwPDB validaton report |
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Full document | 1w6k_full_validation.pdf.gz | 589.4 KB | Display | |
Data in XML | 1w6k_validation.xml.gz | 17.1 KB | Display | |
Data in CIF | 1w6k_validation.cif.gz | 31.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w6/1w6k ftp://data.pdbj.org/pub/pdb/validation_reports/w6/1w6k | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 83392.625 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Plasmid: PPICZB / Production host: PICHIA PASTORIS (fungus) / Strain (production host): GS115 / References: UniProt: P48449, EC: 5.4.99.7 | ||||||
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#2: Sugar | ChemComp-BOG / #3: Chemical | ChemComp-LAN / | #4: Water | ChemComp-HOH / | Compound details | CATALYTIC ACTIVITY: (S)-2,3-EPOXYSQUAL | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.55 Å3/Da / Density % sol: 65.35 % |
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Crystal grow | Details: 25% (W/V) PEG3350 0.4M AMMONIUM ACETATE 0.1M TRIS PH 8.5 10% (V/V) ETHYLENE GLYCOL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.979 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 15, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 79898 / % possible obs: 99.2 % / Observed criterion σ(I): 0 / Redundancy: 5 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 14.4 |
Reflection shell | Resolution: 2→2.09 Å / Redundancy: 5 % / Rmerge(I) obs: 0.49 / Mean I/σ(I) obs: 3.1 / % possible all: 96.3 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2.1→20 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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