+
Open data
-
Basic information
Entry | Database: PDB / ID: 1w3z | ||||||
---|---|---|---|---|---|---|---|
Title | SeMet derivative of BbCRASP-1 from Borrelia Burgdorferi | ||||||
![]() | BBCRASP-1 | ||||||
![]() | COMPLEMENT REGULATOR / COMPLEMENT REGULATOR ACQUIRING SURFACE PROTEIN / LYME BORRELIOSIS / FACTOR H BINDING / TICK / SELENOMETHIONINE | ||||||
Function / homology | Bbcrasp-1 / Bbcrasp-1 / Borrelia lipoprotein paralogus family 54/60 / Borrelia Bbcrasp-1 domain containing protein / Orthogonal Bundle / Mainly Alpha / Complement regulator-acquiring surface protein 1 (CRASP-1) / Complement regulator-acquiring surface protein 1 (CRASP-1)![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cordes, F.S. / Roversi, P. / Goodstadt, L. / Ponting, C. / Kraiczy, P. / Skerka, C. / Kirschfink, M. / Simon, M.M. / Brade, V. / Zipfel, P. ...Cordes, F.S. / Roversi, P. / Goodstadt, L. / Ponting, C. / Kraiczy, P. / Skerka, C. / Kirschfink, M. / Simon, M.M. / Brade, V. / Zipfel, P. / Wallich, R. / Lea, S.M. | ||||||
![]() | ![]() Title: A Novel Fold for the Factor H-Binding Protein Bbcrasp-1 of Borrelia Burgdorferi Authors: Cordes, F.S. / Roversi, P. / Kraiczy, P. / Simon, M.M. / Brade, V. / Jahraus, O. / Wallis, R. / Skerka, C. / Zipfel, P. / Wallich, R. / Lea, S.M. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2004 Title: Crystallisation and Preliminary Crystallographic Analysis of Bbcrasp-1, a Complement Regulator-Acquiring Surface Protein of Borrelia Burgdorferi Authors: Cordes, F.S. / Kraiczy, P. / Roversi, P. / Kraiczy, P. / Skerka, C. / Kirschfink, M. / Simon, M.M. / Brade, V. / Lowe, E. / Zipfel, P. / Wallich, R. / Lea, S.M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 81.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 65 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||||||||||||||
Unit cell |
| ||||||||||||||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
|
-
Components
#1: Protein | Mass: 21572.383 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR DOMAIN, RESIDUES 70-250 Source method: isolated from a genetically manipulated source Details: FACTOR H BINDING DIMER SELENOMETHIONINE DERIVATIVE Source: (gene. exp.) ![]() Strain: ZS7 / Description: TICK ISOLATE GERMANY / Variant: TICK ISOLATE / Plasmid: PGEX-2T / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | Sequence details | RESIDUES 1-25 AND 251 WERE LACKING FROM THE CONSTRUCT | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59 % Description: TWO-WAVELENGTH MAD WITH PEAK AND LOW-ENERGY REMOTE LAMBDA 0.984 A |
---|---|
Crystal grow | pH: 6 / Details: 25% PEG600 200 MM IMIDAZOLE PH 6.0 5% GLYCEROL |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Oct 10, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→73.3 Å / Num. obs: 10849 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 7.6 % / Biso Wilson estimate: 10 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 4 |
Reflection shell | Resolution: 3.2→3.37 Å / Redundancy: 7.9 % / Rmerge(I) obs: 0.42 / Mean I/σ(I) obs: 1.8 / % possible all: 100 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: BABINET SCALING / Bsol: 87 Å2 / ksol: 0.31 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→76.7 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|