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Open data
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Basic information
Entry | Database: PDB / ID: 1w33 | ||||||
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Title | BbCRASP-1 from Borrelia Burgdorferi | ||||||
![]() | BBCRASP-1 | ||||||
![]() | COMPLEMENT REGULATOR / COMPLEMENT REGULATOR ACQUIRING SURFACE PROTEIN / LYME BORRELIOSIS / FACTOR H BINDING / TICK / MEMBRANE PROTEIN | ||||||
Function / homology | Bbcrasp-1 / Bbcrasp-1 / Borrelia lipoprotein paralogus family 54/60 / Borrelia Bbcrasp-1 domain containing protein / Orthogonal Bundle / Mainly Alpha / Complement regulator-acquiring surface protein 1 (CRASP-1) / Complement regulator-acquiring surface protein 1 (CRASP-1)![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Cordes, F.S. / Roversi, P. / Goodstadt, L. / Ponting, C. / Kraiczy, P. / Skerka, C. / Kirschfink, M. / Simon, M.M. / Brade, V. / Zipfel, P. ...Cordes, F.S. / Roversi, P. / Goodstadt, L. / Ponting, C. / Kraiczy, P. / Skerka, C. / Kirschfink, M. / Simon, M.M. / Brade, V. / Zipfel, P. / Wallich, R. / Lea, S.M. | ||||||
![]() | ![]() Title: A Novel Fold for the Factor H-Binding Protein Bbcrasp-1 of Borrelia Burgdorferi Authors: Cordes, F.S. / Roversi, P. / Kraiczy, P. / Simon, M.M. / Brade, V. / Jahraus, O. / Wallis, R. / Skerka, C. / Zipfel, P. / Wallich, R. / Lea, S.M. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2004 Title: Crystallisation and Preliminary Crystallographic Analysis of Bbcrasp-1, a Complement Regulator-Acquiring Surface Protein of Borrelia Burgdorferi Authors: Cordes, F.S. / Kraiczy, P. / Roversi, P. / Kraiczy, P. / Skerka, C. / Kirschfink, M. / Simon, M.M. / Brade, V. / Lowe, E. / Zipfel, P. / Wallich, R. / Lea, S.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 86.3 KB | Display | ![]() |
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PDB format | ![]() | 66.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451 KB | Display | ![]() |
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Full document | ![]() | 476.5 KB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 24.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 21478.592 Da / Num. of mol.: 2 / Fragment: EXTRACELLULAR DOMAIN, RESIDUES 70-250 Source method: isolated from a genetically manipulated source Details: FACTOR H BINDING DIMER Source: (gene. exp.) ![]() Strain: ZS7 / Description: TICK ISOLATE GERMANY / Variant: TICK ISOLATE / Plasmid: PGEX-2T / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | RESIDUES 1-25 AND 251 WERE LACKING FROM THE CONSTRUCT | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 63 % Description: 4 SE SITES FOUND IN HYSS USING PK WAVELENGTH ANOMALOUS PATTERSON |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 / Details: 25% PEG 600 200 MM IMIDAZOLE PH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: ADSC CCD / Detector: CCD / Date: Feb 10, 2003 | |||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 2.7→40.2 Å / Num. obs: 15999 / % possible obs: 95 % / Observed criterion σ(I): 0 / Redundancy: 2.4 % / Biso Wilson estimate: 5.5 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 9.1 | |||||||||
Reflection shell | Resolution: 2.7→2.85 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.257 / Mean I/σ(I) obs: 3 / % possible all: 97.6 |
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Processing
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Refinement | Method to determine structure: OTHER / Resolution: 2.7→19.88 Å / Isotropic thermal model: TNT BCORREL.DAT / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: TNT CSDX_PROTGEO.DAT Details: REFINED IN BUSTER-TNT 1.0.2 THE FIRST 44 RESIDUES (26-69) ARE DISORDERED IN THIS CRYSTAL
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Solvent computation | Solvent model: BABINET SCALING / Bsol: 84 Å2 / ksol: 0.34 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→19.88 Å
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Refine LS restraints |
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