RESIDUES 1-4 IN THE CONSTRUCT USED FOR THE STRUCTURE DETERMINATION ARE FROM THE EXPRESSION VECTOR.
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
TRIPLERESONANCE
1
2
1
NOESY
NMR実験の詳細
Text: STRUCTURAL RESTRAINTS WERE DERIVED FROM 13C AND 15N-EDITED NOESY EXPERIMENTS, J-COUPLINGS, AND H-N RESIDUAL DIPOLAR COUPLINGS.
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試料調製
詳細
内容: 1.0-1.5 MM 15N OR 15N,13C-LABELED PROTEIN, 50 MM NA.PHOSPHATE BUFFER, 150 MM NACL, 0.2 MM DTT
試料状態
イオン強度: 150 mM NaCl mM / pH: 6.8 / 圧: 1 atm / 温度: 295 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
500
1
Bruker DRX
Bruker
DRX
600
2
Bruker DRX
Bruker
DRX
700
3
Bruker DRX
Bruker
DRX
900
4
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解析
NMR software
名称
バージョン
開発者
分類
ARIA1.2, CNS1.1 CNS1.1
CNS1.1
NILGES, BRUNGERETAL.
精密化
NMRView
構造決定
ARIA/CNS
構造決定
精密化
手法: MOLECULAR DYNAMICS, SIMULATED ANNEALING / ソフトェア番号: 1 詳細: THE NMR ENSEMBLE HAS BEEN REFINED IN A SHELL OF WATER MOLECULES.REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.