[English] 日本語
Yorodumi- PDB-1vrw: Crystal structure analysis of plasmodium falciparum enoyl-acyl-ca... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1vrw | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure analysis of plasmodium falciparum enoyl-acyl-carrier-protein reductase with nadh | |||||||||
Components | ENOYL-ACYL CARRIER REDUCTASE | |||||||||
Keywords | OXIDOREDUCTASE / ROSSMANN FOLD / SHORT CHAIN DEHYDROGENASE REDUCTASE / NADH | |||||||||
Function / homology | Function and homology information enoyl-[acyl-carrier-protein] reductase (NADH) activity / fatty acid biosynthetic process / nucleotide binding Similarity search - Function | |||||||||
Biological species | Plasmodium falciparum (malaria parasite P. falciparum) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | |||||||||
Authors | Perozzo, R. / Kuo, M. / Sidhu, A.S. / Valiyaveettil, J.T. / Bittman, R. / Jacobs Jr., W.R. / Fidock, D.A. / Sacchettini, J.C. | |||||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: Structural Elucidation of the Specificity of the Antibacterial Agent Triclosan for Malarial Enoyl Acyl Carrier Protein Reductase Authors: Perozzo, R. / Kuo, M. / Sidhu, A.S. / Valiyaveettil, J.T. / Bittman, R. / Jacobs Jr., W.R. / Fidock, D.A. / Sacchettini, J.C. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1vrw.cif.gz | 125.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1vrw.ent.gz | 99.1 KB | Display | PDB format |
PDBx/mmJSON format | 1vrw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1vrw_validation.pdf.gz | 531.7 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1vrw_full_validation.pdf.gz | 546.1 KB | Display | |
Data in XML | 1vrw_validation.xml.gz | 14.8 KB | Display | |
Data in CIF | 1vrw_validation.cif.gz | 20.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vr/1vrw ftp://data.pdbj.org/pub/pdb/validation_reports/vr/1vrw | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
3 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 38095.957 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium falciparum (malaria parasite P. falciparum) Plasmid: PET28A / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) CODONPLUS-RIL References: UniProt: Q9BH77, enoyl-[acyl-carrier-protein] reductase (NADH) #2: Chemical | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.65 % |
---|---|
Crystal grow | pH: 5.6 / Details: pH 5.60 |
-Data collection
Diffraction | Mean temperature: 120 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.54 |
Detector | Type: MACSCIENCE / Detector: IMAGE PLATE / Date: Jan 11, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→30 Å / Num. obs: 28422 / % possible obs: 94.6 % / Observed criterion σ(I): 0 |
-Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.4→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
| ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→30 Å
|