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- PDB-1vlz: UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS:... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1vlz | ||||||
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Title | UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY | ||||||
![]() | CHEY | ||||||
![]() | SIGNAL TRANSDUCTION PROTEIN / CHEY / RESPONSE REGULATORS / TWO-COMPONENT SYSTEMS | ||||||
Function / homology | ![]() bacterial-type flagellum basal body, C ring / bacterial-type flagellum rotor complex / bacterial-type flagellum-dependent swimming motility / regulation of bacterial-type flagellum-dependent cell motility / aerotaxis / bacterial-type flagellum / histidine phosphotransfer kinase activity / regulation of chemotaxis / thermotaxis / internal peptidyl-lysine acetylation ...bacterial-type flagellum basal body, C ring / bacterial-type flagellum rotor complex / bacterial-type flagellum-dependent swimming motility / regulation of bacterial-type flagellum-dependent cell motility / aerotaxis / bacterial-type flagellum / histidine phosphotransfer kinase activity / regulation of chemotaxis / thermotaxis / internal peptidyl-lysine acetylation / phosphorelay response regulator activity / protein acetylation / phosphorelay signal transduction system / phosphorelay sensor kinase activity / acetyltransferase activity / chemotaxis / magnesium ion binding / signal transduction / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Ganguli, S. / Wang, H. / Matsumura, P. / Volz, K. | ||||||
![]() | ![]() Title: Uncoupled phosphorylation and activation in bacterial chemotaxis. The 2.1-A structure of a threonine to isoleucine mutant at position 87 of CheY. Authors: Ganguli, S. / Wang, H. / Matsumura, P. / Volz, K. #1: ![]() Title: Structural Conservation in the Chey Superfamily Authors: Volz, K. #2: ![]() Title: Crystal Structure of Escherichia Coli Chey Refined at 1.7-Angstrom Resolution Authors: Volz, K. / Matsumura, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 61.8 KB | Display | ![]() |
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PDB format | ![]() | 45.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.8 KB | Display | ![]() |
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Full document | ![]() | 427.7 KB | Display | |
Data in XML | ![]() | 14.1 KB | Display | |
Data in CIF | ![]() | 19.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO A 110 / 2: CIS PROLINE - PRO B 110 | ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.04625, 0.26865, -0.96213), Vector: |
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Components
#1: Protein | Mass: 13993.189 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.54 % |
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Crystal grow | *PLUS Temperature: 37 ℃ / Method: mid-log phase / Details: Matsumura, P., (1984) J. Bacteriol., 160, 36. |
-Data collection
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 15093 / % possible obs: 90.8 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.0457 |
Reflection | *PLUS Highest resolution: 2.04 Å / Lowest resolution: 9999 Å / Num. measured all: 30935 / Rmerge(I) obs: 0.0457 |
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Processing
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Refinement | Resolution: 2.05→10 Å / σ(F): 2 Details: SOLVENT. MOST WATER MOLECULES ARE WELL BEHAVED. A FEW WATERS WITH HIGH TEMPERATURE FACTORS AND CLOSE CONTACTS WITH OTHER WATERS MAY BE PARTIAL SITES. SOLVENT MOLECULES HOH A 214 AND HOH B ...Details: SOLVENT. MOST WATER MOLECULES ARE WELL BEHAVED. A FEW WATERS WITH HIGH TEMPERATURE FACTORS AND CLOSE CONTACTS WITH OTHER WATERS MAY BE PARTIAL SITES. SOLVENT MOLECULES HOH A 214 AND HOH B 203 ARE PROBABLY AMMONIUM IONS.
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Displacement parameters | Biso mean: 16.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.05→10 Å
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Refine LS restraints |
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