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Open data
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Basic information
Entry | Database: PDB / ID: 1vin | ||||||
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Title | BOVINE CYCLIN A3 | ||||||
![]() | CYCLIN A | ||||||
![]() | BINDING PROTEIN / CYCLIN / CELL CYCLE / KINASE-REGULATORY-SUBUNIT | ||||||
Function / homology | ![]() cell cycle G1/S phase transition / mitotic cell cycle phase transition / cyclin-dependent protein serine/threonine kinase regulator activity / cyclin A2-CDK2 complex / regulation of DNA replication / cyclin-dependent protein kinase holoenzyme complex / G2/M transition of mitotic cell cycle / cell division / centrosome / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Noble, M.E.M. / Endicott, J.A.E. / Brown, N.R. | ||||||
![]() | ![]() Title: The crystal structure of cyclin A. Authors: Brown, N.R. / Noble, M.E. / Endicott, J.A. / Garman, E.F. / Wakatsuki, S. / Mitchell, E. / Rasmussen, B. / Hunt, T. / Johnson, L.N. #1: ![]() Title: Two Structures of the Catalytic Domain of Phosphorylase Kinase: An Active Protein Kinase Complexed with Substrate Analogue and Product Authors: Owen, D.J. / Noble, M.E.M. / Garman, E.F. / Papageorgiou, A.C. / Johnson, L.N. #2: ![]() Title: Expression, Purification and Crystallisation of Phosphorylase Kinase Catalytic Domain Authors: Owen, D.J. / Papageorgiou, A.C. / Garman, E.F. / Noble, M.E. / Johnson, L.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64.6 KB | Display | ![]() |
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PDB format | ![]() | 47.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 363.9 KB | Display | ![]() |
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Full document | ![]() | 367.8 KB | Display | |
Data in XML | ![]() | 6.7 KB | Display | |
Data in CIF | ![]() | 10.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 30831.500 Da / Num. of mol.: 1 / Mutation: S171G, DEL(1-170) Source method: isolated from a genetically manipulated source Details: HIS 6 TAGGED AT C-TERMINUS / Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 30 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 8.5 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Num. obs: 17065 / % possible obs: 91.4 % / Redundancy: 4.1 % / Rmerge(I) obs: 0.068 |
Reflection | *PLUS Highest resolution: 2 Å |
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Processing
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Refinement | Resolution: 2→6 Å / Rfactor Rfree: 0.299 / Rfactor Rwork: 0.216 / Rfactor obs: 0.216 / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→6 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.41 |