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- PDB-1umn: Crystal structure of Dps-like peroxide resistance protein (Dpr) f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1umn | ||||||
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Title | Crystal structure of Dps-like peroxide resistance protein (Dpr) from Streptococcus suis | ||||||
![]() | DPS-LIKE PEROXIDE RESISTANCE PROTEIN | ||||||
![]() | PEROXIDE RESISTANCE / IRON-BINDING / FERROXIDASE / DPS-FAMILY / FERRITIN-LIKE | ||||||
Function / homology | ![]() Oxidoreductases; Oxidizing metal ions / ferric iron binding / intracellular iron ion homeostasis / oxidoreductase activity / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kauko, A. / Haataja, S. / Pulliainen, A. / Finne, J. / Papageorgiou, A.C. | ||||||
![]() | ![]() Title: Crystal structure of Streptococcus suis Dps-like peroxide resistance protein Dpr: implications for iron incorporation. Authors: Kauko, A. / Haataja, S. / Pulliainen, A.T. / Finne, J. / Papageorgiou, A.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 401.8 KB | Display | ![]() |
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PDB format | ![]() | 329.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 525.4 KB | Display | ![]() |
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Full document | ![]() | 549.1 KB | Display | |
Data in XML | ![]() | 84 KB | Display | |
Data in CIF | ![]() | 120.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1qghS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 18641.014 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Details: N-TERMINUS TRUNCATED FIRST SEVEN RESIDUES REMOVED. / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Chemical | #4: Chemical | ChemComp-EPE / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 44 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: HANGING DROP METHOD 2.0 UL 10 MG/ML PROTEIN + 2.0 UL RESERVOUR SOLUTION 27 % PEG 400, 0.2 M CACL2, 0.1M HEPES-NAOH, PH 7.4 +16C FEW DAYS |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 15, 2002 / Details: MIRRORS |
Radiation | Monochromator: SI CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→20 Å / Num. obs: 146502 / % possible obs: 97.7 % / Observed criterion σ(I): -3 / Redundancy: 6.3 % / Biso Wilson estimate: 15 Å2 / Rmerge(I) obs: 0.056 / Net I/σ(I): 12 |
Reflection shell | Resolution: 1.95→2.01 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.272 / Mean I/σ(I) obs: 1.7 / % possible all: 91.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QGH Resolution: 1.95→18.08 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 3678334.77 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 53.3696 Å2 / ksol: 0.377455 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.95→18.08 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2.07 Å / Rfactor Rfree error: 0.008 / Total num. of bins used: 6
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Xplor file |
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