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Yorodumi- PDB-1tvn: Cellulase cel5G from Pseudoalteromonas haloplanktis, A family GH ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tvn | ||||||
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Title | Cellulase cel5G from Pseudoalteromonas haloplanktis, A family GH 5-2 enzyme | ||||||
Components | cellulase | ||||||
Keywords | HYDROLASE / Glycoside hydrolase / Clan GH-A / Family 5-2 / Cellulase | ||||||
Function / homology | Function and homology information cellulase / cellulase activity / carbohydrate binding / cell adhesion / carbohydrate metabolic process / calcium ion binding / extracellular region Similarity search - Function | ||||||
Biological species | Pseudoalteromonas haloplanktis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.41 Å | ||||||
Authors | Violot, S. / Haser, R. / Aghajari, N. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2005 Title: Structure of a Full Length Psychrophilic Cellulase from Pseudoalteromonas haloplanktis revealed by X-ray Diffraction and Small Angle X-ray Scattering Authors: Violot, S. / Aghajari, N. / Czjzek, M. / Feller, G. / Sonan, G.K. / Gouet, P. / Gerday, C. / Haser, R. / Receveur-Brechot, V. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2003 Title: Expression, purification, crystallization and preliminary X-ray crystallographic studies of a psychrophilic cellulase from Pseudoalteromonas haloplanktis Authors: Violot, S. / Haser, R. / Sonan, G. / Georlette, D. / Feller, G. / Aghajari, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tvn.cif.gz | 138.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tvn.ent.gz | 108.5 KB | Display | PDB format |
PDBx/mmJSON format | 1tvn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tv/1tvn ftp://data.pdbj.org/pub/pdb/validation_reports/tv/1tvn | HTTPS FTP |
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-Related structure data
Related structure data | 1tvpC 1egzS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31914.918 Da / Num. of mol.: 2 / Fragment: CATALYTIC DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudoalteromonas haloplanktis (bacteria) Gene: celG / Plasmid: pET22b / Production host: Escherichia coli (E. coli) / Strain (production host): Epicurian BL21 (DE3) / References: UniProt: O86099, cellulase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.8 Å3/Da / Density % sol: 33 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.3M tri-sodium citrate dihydrate, 40% (v/v) glycerol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM14 / Wavelength: 0.9559 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Aug 30, 2001 / Details: mirrors |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9559 Å / Relative weight: 1 |
Reflection | Resolution: 1.41→14.008 Å / Num. all: 209086 / Num. obs: 87830 / % possible obs: 95.1 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 2.4 % / Biso Wilson estimate: 11.8 Å2 / Rsym value: 0.043 |
Reflection shell | Resolution: 1.41→1.48 Å / Redundancy: 1.8 % / Num. unique all: 9973 / Rsym value: 0.175 / % possible all: 75.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1EGZ Resolution: 1.41→13.76 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 1740239.82 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 26.2331 Å2 / ksol: 0.392917 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 11.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.41→13.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.41→1.49 Å / Rfactor Rfree error: 0.01 / Total num. of bins used: 6
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Xplor file |
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