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Yorodumi- PDB-1tum: MUTT PYROPHOSPHOHYDROLASE-METAL-NUCLEOTIDE-METAL COMPLEX, NMR, 16... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tum | ||||||
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Title | MUTT PYROPHOSPHOHYDROLASE-METAL-NUCLEOTIDE-METAL COMPLEX, NMR, 16 STRUCTURES | ||||||
Components | MUTATOR MUTT PROTEIN | ||||||
Keywords | MUTATOR PROTEIN / QUATERNARY COMPLEX / NUCLEOSIDE TRIPHOSPHATE PYROPHOSPHOHYDROLASE / MUTT PYROPHOSPHOHYDROLASE-METAL-SUBSTRATE ANALOG COMPLEX | ||||||
Function / homology | Function and homology information dGDP catabolic process / 8-oxo-dGTP diphosphatase / 8-oxo-GDP phosphatase activity / 8-oxo-dGDP phosphatase activity / dGTP catabolic process / 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity / 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity / nucleotide-excision repair / manganese ion binding / DNA replication ...dGDP catabolic process / 8-oxo-dGTP diphosphatase / 8-oxo-GDP phosphatase activity / 8-oxo-dGDP phosphatase activity / dGTP catabolic process / 8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity / 8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity / nucleotide-excision repair / manganese ion binding / DNA replication / DNA repair / magnesium ion binding Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Lin, J. / Abeygunawardana, C. / Frick, D.N. / Bessman, M.J. / Mildvan, A.S. | ||||||
Citation | Journal: Biochemistry / Year: 1997 Title: Solution structure of the quaternary MutT-M2+-AMPCPP-M2+ complex and mechanism of its pyrophosphohydrolase action. Authors: Lin, J. / Abeygunawardana, C. / Frick, D.N. / Bessman, M.J. / Mildvan, A.S. #1: Journal: Biochemistry / Year: 1995 Title: NMR Studies of the Conformations and Location of Nucleotides Bound to the E.Coli Mutt Enzyme Authors: Frick, D.N. / Weber, D.J. / Abeygunawardana, C. / Gittis, A.G. / Bessman, M.J. / Mildvan, A.S. #2: Journal: Biochemistry / Year: 1995 Title: Solution Structure of the Mutt Enzyme, a Nucleoside Triphosphate Pyrophosphohydrolase Authors: Abeygunawardana, C. / Weber, D.J. / Gittis, A.G. / Frick, D.N. / Lin, J. / Miller, A.F. / Bessman, M.J. / Mildvan, A.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tum.cif.gz | 679.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tum.ent.gz | 582.1 KB | Display | PDB format |
PDBx/mmJSON format | 1tum.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tu/1tum ftp://data.pdbj.org/pub/pdb/validation_reports/tu/1tum | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14945.029 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: MUTT-MG(2+)-AMPCPP-MG(2+) COMPLEX / Source: (gene. exp.) Escherichia coli (E. coli) / Strain: K12-I7023 / Plasmid: PET MUTT, T7 PROMOTER / Gene (production host): MUTT / Production host: Escherichia coli (E. coli) / Strain (production host): HMS174 (DE3) References: UniProt: P08337, Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-APC / |
#4: Chemical | ChemComp-CON / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Sample conditions | pH: 7.6 / Temperature: 305 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-Processing
Software |
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NMR software | Name: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement | ||||||||||||
NMR ensemble | Conformers submitted total number: 16 |