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Yorodumi- PDB-1trb: CONVERGENT EVOLUTION OF SIMILAR FUNCTION IN TWO STRUCTURALLY DIVE... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1trb | ||||||
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Title | CONVERGENT EVOLUTION OF SIMILAR FUNCTION IN TWO STRUCTURALLY DIVERGENT ENZYMES | ||||||
Components | THIOREDOXIN REDUCTASE | ||||||
Keywords | OXIDOREDUCTASE(FLAVOENZYME) | ||||||
Function / homology | Function and homology information thioredoxin-disulfide reductase complex / thioredoxin-disulfide reductase / thioredoxin-disulfide reductase (NADPH) activity / removal of superoxide radicals / cell redox homeostasis / flavin adenine dinucleotide binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Kuriyan, J. / Krishna, T.S.R. | ||||||
Citation | Journal: Nature / Year: 1991 Title: Convergent evolution of similar function in two structurally divergent enzymes. Authors: Kuriyan, J. / Krishna, T.S. / Wong, L. / Guenther, B. / Pahler, A. / Williams Jr., C.H. / Model, P. #1: Journal: J.Biol.Chem. / Year: 1989 Title: Crystallization and Preliminary X-Ray Characterization of Thioredoxin Reductase from Escherichia Coli Authors: Kuriyan, J. / Wong, L. / Russel, M. / Model, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1trb.cif.gz | 78.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1trb.ent.gz | 58.4 KB | Display | PDB format |
PDBx/mmJSON format | 1trb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tr/1trb ftp://data.pdbj.org/pub/pdb/validation_reports/tr/1trb | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUE PRO 93 IS A CIS PROLINE. | ||||||||
Details | THE FOLLOWING CRYSTALLOGRAPHIC SYMMETRY OPERATION WILL WILL YIELD COORDINATES FOR THE SECOND CHAIN IN THE DIMER: -0.500000 0.866000 0.000000 0.000000 0.866000 0.500000 0.000000 0.000000 0.000000 0.000000 -1.000000 0.000000 |
-Components
#1: Protein | Mass: 34513.703 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / References: UniProt: P0A9P4, EC: 1.6.4.5 |
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#2: Chemical | ChemComp-FAD / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52.3 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion / PH range low: 7 / PH range high: 6.5 / Details: 'Kuriyan, J.', (1989) J. Biol. Chem., 264, 12752. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2 Å / % possible obs: 95 % / Rmerge(I) obs: 0.005 |
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-Processing
Software |
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Refinement | Rfactor Rwork: 0.177 / Rfactor obs: 0.177 / Highest resolution: 2 Å | ||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||
Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Num. reflection obs: 21106 / σ(I): 2 / Rfactor obs: 0.177 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS | ||||||||||||
Refine LS restraints | *PLUS
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