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Yorodumi- PDB-1tde: CRYSTAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN REDUCTASE REFIN... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tde | ||||||
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Title | CRYSTAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN REDUCTASE REFINED AT 2 ANGSTROM RESOLUTION: IMPLICATIONS FOR A LARGE CONFORMATIONAL CHANGE DURING CATALYSIS | ||||||
Components | THIOREDOXIN REDUCTASE | ||||||
Keywords | OXIDOREDUCTASE(FLAVOENZYME) | ||||||
Function / homology | Function and homology information thioredoxin-disulfide reductase complex / thioredoxin-disulfide reductase / thioredoxin-disulfide reductase (NADPH) activity / removal of superoxide radicals / cell redox homeostasis / flavin adenine dinucleotide binding / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.1 Å | ||||||
Authors | Waksman, G. / Krishna, T.S.R. / Williams Junior, C.H. / Kuriyan, J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1994 Title: Crystal structure of Escherichia coli thioredoxin reductase refined at 2 A resolution. Implications for a large conformational change during catalysis. Authors: Waksman, G. / Krishna, T.S. / Williams Jr., C.H. / Kuriyan, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tde.cif.gz | 89.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tde.ent.gz | 74.5 KB | Display | PDB format |
PDBx/mmJSON format | 1tde.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/td/1tde ftp://data.pdbj.org/pub/pdb/validation_reports/td/1tde | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 93 |
-Components
#1: Protein | Mass: 34143.340 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / References: UniProt: P0A9P4, EC: 1.6.4.5 |
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#2: Chemical | ChemComp-FAD / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.43 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion / Details: 'Kuriyan, J.', (1989) J. Biol. Chem., 264, 12752. / PH range low: 7 / PH range high: 6.5 | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 20 Å / Num. obs: 19643 / % possible obs: 85 % / Num. measured all: 54700 / Rmerge(I) obs: 0.057 |
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-Processing
Software |
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Refinement | Resolution: 2.1→6 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.1→6 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.192 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.3 |