[English] 日本語
Yorodumi
- PDB-1tof: THIOREDOXIN H (OXIDIZED FORM), NMR, 23 STRUCTURES -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 1tof
TitleTHIOREDOXIN H (OXIDIZED FORM), NMR, 23 STRUCTURES
ComponentsTHIOREDOXIN H
KeywordsELECTRON TRANSPORT / OXIDOREDUCTASE
Function / homology
Function and homology information


Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / Glutaredoxin / Glutaredoxin / Thioredoxin-like superfamily / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Biological speciesChlamydomonas reinhardtii (plant)
MethodSOLUTION NMR
AuthorsMittard, V. / Blackledge, M.J. / Stein, M. / Jacquot, J.-P. / Marion, D. / Lancelin, J.-M.
Citation
Journal: Eur.J.Biochem. / Year: 1997
Title: NMR solution structure of an oxidised thioredoxin h from the eukaryotic green alga Chlamydomonas reinhardtii.
Authors: Mittard, V. / Blackledge, M.J. / Stein, M. / Jacquot, J.P. / Marion, D. / Lancelin, J.M.
#1: Journal: Plant Mol.Biol. / Year: 1995
Title: Chlamydomonas Reinhardtii Thioredoxins: Structure of the Genes Coding for the Chloroplastic M and Cytosolic H Isoforms; Expression in Escherichia Coli of the Recombinant Proteins, Purification ...Title: Chlamydomonas Reinhardtii Thioredoxins: Structure of the Genes Coding for the Chloroplastic M and Cytosolic H Isoforms; Expression in Escherichia Coli of the Recombinant Proteins, Purification and Biochemical Properties
Authors: Stein, M. / Jacquot, J.P. / Jeannette, E. / Decottignies, P. / Hodges, M. / Lancelin, J.M. / Mittard, V. / Schmitter, J.M. / Miginiac-Maslow, M.
#2: Journal: Eur.J.Biochem. / Year: 1995
Title: 1H, 13C, 15N-NMR Resonance Assignments of Oxidized Thioredoxin H from the Eukaryotic Green Alga Chlamydomonas Reinhardtii Using New Methods Based on Two-Dimensional Triple-Resonance NMR ...Title: 1H, 13C, 15N-NMR Resonance Assignments of Oxidized Thioredoxin H from the Eukaryotic Green Alga Chlamydomonas Reinhardtii Using New Methods Based on Two-Dimensional Triple-Resonance NMR Spectroscopy and Computer-Assisted Backbone Assignment
Authors: Mittard, V. / Morelle, N. / Brutscher, B. / Simorre, J.P. / Marion, D. / Stein, M. / Jacquot, J.P. / Lirsac, P.N. / Lancelin, J.M.
History
DepositionMay 30, 1996Processing site: BNL
Revision 1.0Dec 7, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_nmr_software / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _pdbx_nmr_software.name
Revision 1.4Oct 30, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_entry_details / pdbx_modification_feature

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: THIOREDOXIN H


Theoretical massNumber of molelcules
Total (without water)11,7281
Polymers11,7281
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)23 / -
Representative

-
Components

#1: Protein THIOREDOXIN H / HTRX / HCH1 / CH1


Mass: 11727.534 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Chlamydomonas reinhardtii (plant) / Strain: CW15 / Cellular location: CYTOPLASM / Gene: THIOREDOXIN H CDNA (X78822 EMB / Plasmid: PET-3D / Species (production host): Escherichia coli / Gene (production host): THIOREDOXIN H CDNA (X78822,EMBL) / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P80028
Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: SOLUTION NMR

-
Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

-
Processing

NMR softwareName: Discover / Developer: BIOSYM / Classification: refinement
NMR ensembleConformers submitted total number: 23

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more