[English] 日本語
![](img/lk-miru.gif)
- PDB-1tid: Crystal Structures of the ADP and ATP bound forms of the Bacillus... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1tid | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structures of the ADP and ATP bound forms of the Bacillus Anti-sigma factor SpoIIAB in complex with the Anti-anti-sigma SpoIIAA: Poised for phosphorylation complex with ATP, crystal form I | ||||||
![]() |
| ||||||
![]() | TRANSCRIPTION / SpoIIAB / SpoIIAA / anti-sigma / anti-anti-sigma / sporulation / serine kinase | ||||||
Function / homology | ![]() asexual sporulation / negative regulation of sporulation resulting in formation of a cellular spore / anti-sigma factor antagonist activity / antisigma factor binding / sigma factor antagonist activity / sporulation resulting in formation of a cellular spore / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Masuda, S. / Murakami, K.S. / Wang, S. / Olson, C.A. / Donigan, J. / Leon, F. / Darst, S.A. / Campbell, E.A. | ||||||
![]() | ![]() Title: Crystal Structures of the ADP and ATP Bound Forms of the Bacillus Anti-sigma Factor SpoIIAB in Complex with the Anti-anti-sigma SpoIIAA. Authors: Masuda, S. / Murakami, K.S. / Wang, S. / Olson, C.A. / Donigian, J. / Leon, F. / Darst, S.A. / Campbell, E.A. | ||||||
History |
| ||||||
Remark 999 | SEQUENCE THE DISCREPANCIES IN BOTH CHAINS ARE DUE TO STRAIN VARIATION. |
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 114.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 89.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 24.4 KB | Display | |
Data in CIF | ![]() | 32.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1th8C ![]() 1thnC ![]() 1tilC ![]() 1h4yS ![]() 1l0oS C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | the asymmetric unit contains one biological assembly of 2AB/2AA (a tetramer of 2 heterodimers) |
-
Components
#1: Protein | Mass: 15410.411 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: SPOIIAB / Plasmid: pET21a / Species (production host): Escherichia coli / Production host: ![]() ![]() #2: Protein | Mass: 13269.778 Da / Num. of mol.: 2 / Mutation: S58A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: SPOIIAA / Plasmid: pET21a / Species (production host): Escherichia coli / Production host: ![]() ![]() #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.87 Å3/Da / Density % sol: 57.1 % |
---|---|
Crystal grow | Temperature: 295.5 K / Method: vapor diffusion, hanging drop / pH: 9.7 Details: 0.1M CHESS, 2-5% DMF, 2.0 M lithium sulfate, pH 9.7, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 8, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→30 Å / Num. all: 25904 / Num. obs: 24765 / % possible obs: 95.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.5 % / Rsym value: 0.058 / Net I/σ(I): 18.5 |
Reflection shell | Resolution: 2.5→2.59 Å / Mean I/σ(I) obs: 5.8 / Num. unique all: 2682 / Rsym value: 0.19 / % possible all: 94.5 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: pdb entry ID 1L0O, pdb entry ID 1H4Y Resolution: 2.5→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→30 Å
| |||||||||||||||||||||||||
Refine LS restraints |
|