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- PDB-1t2y: NMR solution structure of the protein part of Cu6-Neurospora crassa MT -
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Open data
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Basic information
Entry | Database: PDB / ID: 1t2y | ||||||
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Title | NMR solution structure of the protein part of Cu6-Neurospora crassa MT | ||||||
![]() | Metallothionein | ||||||
![]() | METAL BINDING PROTEIN / protein fold / no secondary structural elements | ||||||
Function / homology | Metallothionein domain superfamily / metal ion binding / Metallothionein![]() | ||||||
Method | SOLUTION NMR / the hybrid distance geometry-dynamical simulated annealing protocol | ||||||
![]() | Cobine, P.A. / McKay, R.T. / Zangger, K. / Dameron, C.T. / Armitage, I.M. | ||||||
![]() | ![]() Title: Solution structure of Cu metallothionein from the fungus Neurospora crassa Authors: Cobine, P.A. / McKay, R.T. / Zangger, K. / Dameron, C.T. / Armitage, I.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 11 KB | Display | ![]() |
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PDB format | ![]() | 7.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 238.5 KB | Display | ![]() |
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Full document | ![]() | 238.3 KB | Display | |
Data in XML | ![]() | 2.1 KB | Display | |
Data in CIF | ![]() | 2.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 2235.421 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence of the peptide occurs naturally in the fungus neurospora crassa. References: UniProt: P02807 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques |
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Sample preparation
Details | Contents: 0.5 mM Neurospora crassa MT, 20 mM phosphate buffer, 0.1 mM 2,2-dimethyl-2-silapentane-5-sulfonate, 0.02% NaN3, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 20 mM KPi / pH: 6.5 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: the hybrid distance geometry-dynamical simulated annealing protocol Software ordinal: 1 Details: the structure was calculated with 152 NOEs and 13 dihedral angle restraints | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformers submitted total number: 1 |