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- PDB-1tjb: Crystal Structure of a High Affinity Lanthanide-Binding Peptide (LBT) -
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Open data
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Basic information
Entry | Database: PDB / ID: 1tjb | ||||||
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Title | Crystal Structure of a High Affinity Lanthanide-Binding Peptide (LBT) | ||||||
![]() | Lanthanide-Binding Peptide | ||||||
![]() | DE NOVO PROTEIN / Lanthanide-Based Resonance Energy Transfer / Fluorescence / EF-Hand / Troponin Based Design / Lanthanide Binding Tag | ||||||
Function / homology | TERBIUM(III) ION![]() | ||||||
Method | ![]() | ||||||
![]() | Nitz, M. / Sherawat, M. / Franz, K.J. / Peisach, E. / Allen, K.N. / Imperiali, B. | ||||||
![]() | ![]() Title: Structural Origin of the High Affinity of a Chemically Evolved Lanthanide-Binding Peptide Authors: Nitz, M. / Sherawat, M. / Franz, K.J. / Peisach, E. / Allen, K.N. / Imperiali, B. #1: ![]() Title: Lanthanide-binding tags as versatile protein coexpression probes Authors: Franz, K.J. / Nitz, M. / Imperiali, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 15.3 KB | Display | ![]() |
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PDB format | ![]() | 11.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 406.2 KB | Display | ![]() |
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Full document | ![]() | 406.2 KB | Display | |
Data in XML | ![]() | 4.1 KB | Display | |
Data in CIF | ![]() | 5.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The solution assembly is a monomer |
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Components
#1: Protein/peptide | Mass: 1986.036 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: Peptide prepared by automated solid phase peptide synthesis #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: t-Butanol, Terbium Chloride, sodium chloride, tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jul 26, 2003 / Details: Osmic Mirrors |
Radiation | Monochromator: OSMIC MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→55 Å / Num. all: 2315 / Num. obs: 2315 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.6 % / Biso Wilson estimate: 6.8 Å2 / Rmerge(I) obs: 0.107 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 3.8 % / Rmerge(I) obs: 0.171 / Mean I/σ(I) obs: 7.8 / Num. unique all: 212 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: SAD PHASING / Resolution: 2→17.18 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 14.77 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→17.18 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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Xplor file |
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