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Open data
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Basic information
Entry | Database: PDB / ID: 1smz | ||||||
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Title | Structure of Transportan in phospholipid bicellar solution | ||||||
![]() | Transportan in bicellar solution with [DMPC]/[DHPC]=0.33 | ||||||
![]() | TRANSPORT PROTEIN | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Barany-Wallje, E. / Andersson, A. / Maler, L. / Graslund, A. | ||||||
![]() | ![]() Title: NMR solution structure and position of transportan in neutral phospholipid bicelles Authors: Andersson, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 210 KB | Display | ![]() |
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PDB format | ![]() | 175.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 340 KB | Display | ![]() |
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Full document | ![]() | 457.8 KB | Display | |
Data in XML | ![]() | 16.4 KB | Display | |
Data in CIF | ![]() | 24.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 2845.470 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The protein was chemically synthesized. This sequence does not occur naturally. |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
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Sample preparation
Details | Contents: 1mM Transportan in neutral bicelles, [lipid]=300mM [DMPC]/[PHPC]=0.33, 50mM phosphate buffer, 90% H2O, 10% D20 Solvent system: 90% H2O, 10% D20 |
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Sample conditions | pH: 5.6 / Pressure: ambient / Temperature: 310 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: The structures are based on 220 NOE-derived distance constraints | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: The submitted conformer models are the 25 structures with the lowest energy Conformers calculated total number: 60 / Conformers submitted total number: 25 |