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Yorodumi- PDB-1sk5: The ultra-high resolution structure of d(CTTTTAAAAG)2: modulation... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sk5 | ||||||||||||||||||
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Title | The ultra-high resolution structure of d(CTTTTAAAAG)2: modulation of bending by T-A steps and its role in DNA recognition | ||||||||||||||||||
Components | 5'-D(*Keywords | DNA / DNA/DNA double helix / Popypurine Tract Sequence of HIV-1 | Function / homology | DNA | Function and homology information Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 0.89 Å | Authors | Han, G.W. / Langs, D. / Kopka, M.L. / Dickerson, R.E. | Citation | Journal: To be Published Title: The ultra-high resolution structure of d(CTTTTAAAAG)2: modulation of bending by T-A steps and its role in DNA recognition Authors: Han, G.W. / Langs, D. / Kopka, M.L. / Dickerson, R.E. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2001 Title: Direct-methods determination of an RNA/DNA hybrid decamer at 1.15A Resolution Authors: Han, G.W. #2: Journal: J.Mol.Biol. / Year: 2003 Title: An Unusual Sugar Conformation in the Structure of an RNA/DNA Decamer of the Polypurine Tract May Affect Recognition by Rnase H Authors: Kopka, M.L. / Lavelle, L. / Han, G.W. / Ng, H.L. / Dickerson, R.E. #3: Journal: J.Mol.Biol. / Year: 1997 Title: Structure of a DNA analog of the primer for HIV-1 RT second strand synthesis Authors: Han, G.W. / Kopka, M.L. / Cascio, D. / Grzeskowiak, K. / Dickerson, R.E. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sk5.cif.gz | 41.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sk5.ent.gz | 29.3 KB | Display | PDB format |
PDBx/mmJSON format | 1sk5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sk5_validation.pdf.gz | 325.7 KB | Display | wwPDB validaton report |
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Full document | 1sk5_full_validation.pdf.gz | 325.9 KB | Display | |
Data in XML | 1sk5_validation.xml.gz | 1.5 KB | Display | |
Data in CIF | 1sk5_validation.cif.gz | 2.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sk/1sk5 ftp://data.pdbj.org/pub/pdb/validation_reports/sk/1sk5 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3043.028 Da / Num. of mol.: 2 / Source method: obtained synthetically Details: SYNTHESIZED BY THE SOLID-PHASE PHOSPHORAMIDATE METHOD ON AN EPPENDORF ECOSYN D300 SYNTHESIZER #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.14 % | ||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop Details: Calcium acetate, Spermine, MPD, VAPOR DIFFUSION, HANGING DROP, temperature 269K | ||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Type: NSLS / Wavelength: 0.9 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 0.89→50 Å / Num. all: 32054 / Num. obs: 32054 / Redundancy: 10.2 % / Rsym value: 0.073 |
Reflection shell | Resolution: 0.89→0.92 Å / Mean I/σ(I) obs: 3 / Rsym value: 0.573 |
-Processing
Software |
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Refinement | Starting model: NONE Resolution: 0.89→50 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 0.89→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 0.89→0.94 Å / Rfactor Rwork: 0.235 |