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Yorodumi- PDB-1sbp: 1.7 ANGSTROMS REFINED STRUCTURE OF SULFATE-BINDING PROTEIN INVOLV... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sbp | ||||||
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Title | 1.7 ANGSTROMS REFINED STRUCTURE OF SULFATE-BINDING PROTEIN INVOLVED IN ACTIVE TRANSPORT AND NOVEL MODE OF SULFATE BINDING | ||||||
Components | SULFATE-BINDING PROTEIN | ||||||
Keywords | BINDING PROTEIN | ||||||
Function / homology | Function and homology information sulfur compound binding / ABC-type sulfate transporter activity / periplasmic space Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.7 Å | ||||||
Authors | Sack, J.S. / Quiocho, F.A. | ||||||
Citation | Journal: Protein Sci. / Year: 1993 Title: Dominant role of local dipoles in stabilizing uncompensated charges on a sulfate sequestered in a periplasmic active transport protein. Authors: He, J.J. / Quiocho, F.A. #1: Journal: J.Biol.Chem. / Year: 1991 Title: Engineered Interdomain Disulfide in the Periplasmic Receptor for Sulfate Transport Reduces Flexibility. Site-Directed Mutagenesis and Ligand-Binding Studies Authors: Jacobson, B.L. / He, J.J. / Vermersch, P.S. / Lemon, D.D. / Quiocho, F.A. #2: Journal: J.Mol.Biol. / Year: 1988 Title: The 2 Angstroms Resolution Structure of the Sulfate-Binding Protein Involved in Active Transport in Salmonella Typhimurium Authors: Pflugrath, J.W. / Quiocho, F.A. #3: Journal: J.Mol.Biol. / Year: 1988 Title: Sulfate-Binding Protein Dislikes Protonated Oxyacids. A Molecular Explanation Authors: Jacobson, B.L. / Quiocho, F.A. #4: Journal: Nature / Year: 1985 Title: Sulphate Sequestered in the Sulphate-Binding Protein of Salmonella Typhimurium is Bound Solely by Hydrogen Bonds Authors: Pflugrath, J.W. / Quiocho, F.A. #5: Journal: J.Biol.Chem. / Year: 1980 Title: Amino Acid Sequence of the Sulfate-Binding Protein from Salmonella Typhimurium Lt2 Authors: Isihara, H. / Hogg, R.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sbp.cif.gz | 73.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sbp.ent.gz | 55.5 KB | Display | PDB format |
PDBx/mmJSON format | 1sbp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sb/1sbp ftp://data.pdbj.org/pub/pdb/validation_reports/sb/1sbp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34529.531 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / References: UniProt: P02906 |
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#2: Chemical | ChemComp-SO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.13 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 4.2 / Method: vapor diffusion / Details: Wilson, D.K., (1988) J.Mol.Biol., 200, 613. | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Rfactor obs: 0.178 / Highest resolution: 1.7 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 1.7 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.7 Å / Rfactor obs: 0.178 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |