+
Open data
-
Basic information
Entry | Database: PDB / ID: 1s0w | ||||||
---|---|---|---|---|---|---|---|
Title | 1b Lactamse/ b Lactamase Inhibitor | ||||||
![]() |
| ||||||
![]() | HYDROLASE / PROTEIN-PROTEIN COMPLEX / TEM-1 BETA-LACTAMASE / BETA-LACTAMASE INHIBITOR PROTEIN / BLIP / Israel Structural Proteomics Center / ISPC / Structural Genomics | ||||||
Function / homology | ![]() regulation of beta-lactamase activity / beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / response to antibiotic / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Schreiber, G. / Reichman, D. / Israel Structural Proteomics Center (ISPC) | ||||||
![]() | ![]() Title: The modular architecture of protein-protein binding interfaces. Authors: Reichmann, D. / Rahat, O. / Albeck, S. / Meged, R. / Dym, O. / Schreiber, G. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 179.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 141 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 462.1 KB | Display | |
Data in XML | ![]() | 35.7 KB | Display | |
Data in CIF | ![]() | 50.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1xxmC ![]() 1jtgS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data | |
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 28941.994 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 17480.396 Da / Num. of mol.: 2 / Mutation: F1142A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 49.93 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 293 K / Method: microbatch / pH: 7.5 Details: PEG 8000, Dioxane, pH 7.5, Microbatch, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: batch method | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 120 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40 Å / Num. obs: 40572 / % possible obs: 96.9 % / Redundancy: 7 % / Biso Wilson estimate: 27.1 Å2 / Rmerge(I) obs: 0.05 / Rsym value: 0.057 / Net I/σ(I): 22 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 5 % / Rmerge(I) obs: 0.189 / Mean I/σ(I) obs: 20.3 / Num. unique all: 3657 / Rsym value: 0.04 / % possible all: 89.9 |
Reflection | *PLUS Num. obs: 40514 / % possible obs: 97.2 % / Num. measured all: 498302 / Rmerge(I) obs: 0.057 |
Reflection shell | *PLUS % possible obs: 89.9 % / Rmerge(I) obs: 0.203 / Mean I/σ(I) obs: 6 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1jtg Resolution: 2.3→40 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→40 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
LS refinement shell | Resolution: 2.3→2.32 Å /
| ||||||||||||||||||||
Refinement | *PLUS Highest resolution: 3 Å / % reflection Rfree: 10 % / Rfactor Rfree: 0.262 / Rfactor Rwork: 0.213 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |