+Open data
-Basic information
Entry | Database: PDB / ID: 1s0u | ||||||
---|---|---|---|---|---|---|---|
Title | eIF2gamma apo | ||||||
Components | Translation initiation factor 2 gamma subunit | ||||||
Keywords | TRANSLATION / translation initiation / GTPase / EF-1A / tRNA / eIF2 | ||||||
Function / homology | Function and homology information protein-synthesizing GTPase / formation of translation preinitiation complex / positive regulation of translational fidelity / translation elongation factor activity / translation initiation factor activity / GTPase activity / GTP binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Methanocaldococcus jannaschii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.4 Å | ||||||
Authors | Roll-Mecak, A. / Alone, P. / Cao, C. / Dever, T.E. / Burley, S.K. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: X-ray Structure of Translation Initiation Factor eIF2gamma: IMPLICATIONS FOR tRNA AND eIF2alpha BINDING. Authors: Roll-Mecak, A. / Alone, P. / Cao, C. / Dever, T.E. / Burley, S.K. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1s0u.cif.gz | 85.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1s0u.ent.gz | 66.4 KB | Display | PDB format |
PDBx/mmJSON format | 1s0u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s0/1s0u ftp://data.pdbj.org/pub/pdb/validation_reports/s0/1s0u | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 44151.051 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanocaldococcus jannaschii (archaea) Gene: EIF2G, MJ1261 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q58657 |
---|---|
#2: Chemical | ChemComp-ZN / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.1 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 100mM Hepes, 10% PEG 6K, 5% v/v methyl pentanediol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 108 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.97884 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 15, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97884 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→22 Å / Num. obs: 15988 / % possible obs: 98.4 % / Observed criterion σ(F): 2 |
Reflection shell | Resolution: 2.4→2.49 Å / % possible all: 84.2 |
Reflection | *PLUS Lowest resolution: 22 Å / Redundancy: 6 % / Rmerge(I) obs: 0.058 |
Reflection shell | *PLUS % possible obs: 84.2 % / Rmerge(I) obs: 0.171 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: SAD / Resolution: 2.4→22 Å / σ(F): 2 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→22 Å
| ||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 22 Å / % reflection Rfree: 10 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
|