[English] 日本語
Yorodumi- PDB-1s01: LARGE INCREASES IN GENERAL STABILITY FOR SUBTILISIN BPN(PRIME) TH... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1s01 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | LARGE INCREASES IN GENERAL STABILITY FOR SUBTILISIN BPN(PRIME) THROUGH INCREMENTAL CHANGES IN THE FREE ENERGY OF UNFOLDING | |||||||||
Components | Subtilisin BPN' | |||||||||
Keywords | HYDROLASE / HYDROLASE (SERINE PROTEINASE) | |||||||||
Function / homology | Function and homology information subtilisin / sporulation resulting in formation of a cellular spore / fibrinolysis / serine-type endopeptidase activity / proteolysis / extracellular region / metal ion binding Similarity search - Function | |||||||||
Biological species | Bacillus amyloliquefaciens (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 1.7 Å | |||||||||
Authors | Whitlow, M. / Howard, A.J. / Wood, J.F. | |||||||||
Citation | Journal: Biochemistry / Year: 1989 Title: Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding. Authors: Pantoliano, M.W. / Whitlow, M. / Wood, J.F. / Dodd, S.W. / Hardman, K.D. / Rollence, M.L. / Bryan, P.N. #1: Journal: J.Am.Chem.Soc. / Year: 1990 Title: Enzymes in Organic Synthesis. Use of Subtilisin and a Highly Stable Mutant Derived from Multiple Site-Specific Mutations Authors: Wong, C.-H. / Chen, S.-T. / Hennen, W.J. / Bibbs, J.A. / Wang, Y.-F. / Liu, J.L.-C. / Pantoliano, M.W. / Whitlow, M. / Bryan, P.N. #2: Journal: Biochemistry / Year: 1988 Title: The Engineering of Binding Affinity at Metal Ion Binding Sites for the Stabilization of Proteins. Subtilisin as a Test Case Authors: Pantoliano, M.W. / Whitlow, M. / Wood, J.F. / Rollence, M.L. / Finzel, B.C. / Gilliland, G.L. / Poulos, T.L. / Bryan, P.N. #3: Journal: Crit.Rev.Biotechnol. / Year: 1988 Title: Engineering Thermostability in Subtilisin Bpn(Prime) by in Vitro Mutagenesis Authors: Rollence, M.L. / Filpula, D. / Pantoliano, M.W. / Bryan, P.N. #4: Journal: Proteins / Year: 1986 Title: Proteases of Enhanced Stability. Characterization of a Thermostable Variant of Subtilisin Authors: Bryan, P.N. / Rollence, M.L. / Pantoiano, M.W. / Wood, J. / Finzel, B.C. / Gilliland, G.L. / Howard, A.J. / Poulos, T.L. #5: Journal: Biochem.Biophys.Res.Commun. / Year: 1971 Title: Atomic Coordinates for Subtilisin Bpn(or Novo) Authors: Alden, R.A. / Birktoft, J.J. / Kraut, J. / Robertus, J.D. / Wright, C.S. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1s01.cif.gz | 63.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1s01.ent.gz | 49.3 KB | Display | PDB format |
PDBx/mmJSON format | 1s01.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/s0/1s01 ftp://data.pdbj.org/pub/pdb/validation_reports/s0/1s01 | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: RESIDUE PRO 168 IS A CIS PROLINE. 2: RESIDUE 206 APPEARS TO BE CYSTEINE PERSULFIDE. ELECTRON DENSITY FOUND BEYOND THE PERSULFIDE HAS BEEN MODELLED AS A PARTIALLY OCCUPIED CARBON ATOM. THE ADDITIONAL SULFUR AND CARBON ARE PRESENTED ON ...2: RESIDUE 206 APPEARS TO BE CYSTEINE PERSULFIDE. ELECTRON DENSITY FOUND BEYOND THE PERSULFIDE HAS BEEN MODELLED AS A PARTIALLY OCCUPIED CARBON ATOM. THE ADDITIONAL SULFUR AND CARBON ARE PRESENTED ON *HETATM* RECORDS AS HET GROUP * CS*. THE EXACT CHEMICAL IDENTITY OF THIS GROUP IS, HOWEVER, UNKNOWN. SEE THE PAPER CITED ON THE *JRNL* RECORDS ABOVE. |
-Components
#1: Protein | Mass: 27543.602 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Gene: apr / Production host: Bacillus amyloliquefaciens (bacteria) / References: UniProt: P00782, subtilisin | ||||
---|---|---|---|---|---|
#2: Chemical | ChemComp-CA / | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Compound details | RESIDUE 206 APPEARS TO BE CYSTEINE PERSULFIDE. ELECTRON DENSITY FOUND BEYOND THE PERSULFIDE HAS ...RESIDUE 206 APPEARS TO BE CYSTEINE PERSULFIDE | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.65 % |
---|---|
Crystal grow | *PLUS Method: vapor diffusion, hanging drop |
Components of the solutions | *PLUS Conc.: 55 % / Common name: acetone |
-Processing
Software | Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.7→10 Å /
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→10 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 10 Å / Num. reflection obs: 17421 / Rfactor obs: 0.152 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |