+
Open data
-
Basic information
Entry | Database: PDB / ID: 1rvh | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | SOLUTION STRUCTURE OF THE DNA DODECAMER GCAAAATTTTGC | ||||||||||||||||||
![]() | 5'-D(*![]() DNA / DOUBLE HELIX | Function / homology | DNA / DNA (> 10) | ![]() Method | SOLUTION NMR / simulated annealing, for details see (Padrta et al. (2002) J. Biomol. NMR 24, 1-24) | ![]() Stefl, R. / Wu, H. / Ravindranathan, S. / Sklenar, V. / Feigon, J. | ![]() ![]() Title: DNA A-tract bending in three dimensions: Solving the dA4T4 vs. dT4A4 conundrum. Authors: Stefl, R. / Wu, H. / Ravindranathan, S. / Sklenar, V. / Feigon, J. History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 140.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 114.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: DNA chain | Mass: 3661.416 Da / Num. of mol.: 2 / Source method: obtained synthetically |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||||||
NMR details | Text: THIS STRUCTURE WAS DETERMINED USING RESIDUAL DIPOLAR COUPLINGS |
-
Sample preparation
Details |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Sample conditions | Ionic strength: 25 mM NH4Cl / pH: 6 / Pressure: ambient / Temperature: 288 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
|
-
Processing
NMR software | Name: ![]() |
---|---|
Refinement | Method: simulated annealing, for details see (Padrta et al. (2002) J. Biomol. NMR 24, 1-24) Software ordinal: 1 Details: the structures are based on a total of 870 restraints, 586 are NOE-derived distance restraints, 160 torsion angle restraints, 24 pseudorotation phase angle restraints, 24 distance restraints ...Details: the structures are based on a total of 870 restraints, 586 are NOE-derived distance restraints, 160 torsion angle restraints, 24 pseudorotation phase angle restraints, 24 distance restraints from W-C hydrogen bonds. |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 9 |