+Open data
-Basic information
Entry | Database: PDB / ID: 1ruz | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | 1918 H1 Hemagglutinin | |||||||||
Components | (hemagglutinin) x 2 | |||||||||
Keywords | VIRAL PROTEIN / Hemagglutinin / INFLUENZA A VIRUS | |||||||||
Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / membrane => GO:0016020 / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane Similarity search - Function | |||||||||
Biological species | Influenza A virus | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | |||||||||
Authors | Skehel, J.J. / Gamblin, S.J. / Haire, L.F. / Russell, R.J. / Stevens, D.J. / Xiao, B. / Ha, Y. / Vasisht, N. / Steinhauer, D.A. / Daniels, R.S. | |||||||||
Citation | Journal: Science / Year: 2004 Title: The structure and receptor binding properties of the 1918 influenza hemagglutinin. Authors: Gamblin, S.J. / Haire, L.F. / Russell, R.J. / Stevens, D.J. / Xiao, B. / Ha, Y. / Vasisht, N. / Steinhauer, D.A. / Daniels, R.S. / Elliot, A. / Wiley, D.C. / Skehel, J.J. | |||||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1ruz.cif.gz | 305 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1ruz.ent.gz | 248.1 KB | Display | PDB format |
PDBx/mmJSON format | 1ruz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ruz_validation.pdf.gz | 453.5 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1ruz_full_validation.pdf.gz | 509.5 KB | Display | |
Data in XML | 1ruz_validation.xml.gz | 37.7 KB | Display | |
Data in CIF | 1ruz_validation.cif.gz | 57 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ru/1ruz ftp://data.pdbj.org/pub/pdb/validation_reports/ru/1ruz | HTTPS FTP |
-Related structure data
Related structure data | 1ru7SC 1ruyC 1rv0C 1rvtC 1rvxC 1rvzC S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 36125.465 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Details: Hemagglutinin HA1 chain Source: (natural) Influenza A virus (A/South Carolina/1/18 (H1N1)) Genus: Influenzavirus A / Cell: Vaccinia virus infected CV1 cells / Species: Influenza A virus / Strain: A-SOUTH CAROLINA-1-18 (H1N1) / References: UniProt: Q9WFZ1, UniProt: Q9WFX3*PLUS #2: Protein | Mass: 18212.092 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Details: Hemagglutinin HA2 chain Source: (natural) Influenza A virus (A/South Carolina/1/18 (H1N1)) Genus: Influenzavirus A / Cell: Vaccinia virus infected CV1 cells / Species: Influenza A virus / Strain: A-SOUTH CAROLINA-1-18 (H1N1) / References: UniProt: Q9WFZ1, UniProt: Q9WFX3*PLUS #3: Sugar | ChemComp-NDG / #4: Sugar | #5: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 3.46 Å3/Da / Density % sol: 64.43 % |
---|---|
Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: PEG3350, TrisHCl, Bis tris propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX14.2 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 20, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.9→20 Å / Num. all: 51196 / Num. obs: 49134 / % possible obs: 96.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 19 % / Rmerge(I) obs: 0.085 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 2.9→3.1 Å / Rmerge(I) obs: 0.574 / Mean I/σ(I) obs: 1.8 / % possible all: 88.4 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1RU7 Resolution: 2.9→20 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→20 Å
|