[English] 日本語
![](img/lk-miru.gif)
- PDB-1rta: CRYSTAL STRUCTURE DISPOSITION OF THYMIDYLIC ACID TETRAMER IN COMP... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1rta | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE DISPOSITION OF THYMIDYLIC ACID TETRAMER IN COMPLEX WITH RIBONUCLEASE A | ||||||
![]() |
| ||||||
![]() | HYDROLASE/DNA / PROTEIN-DNA COMPLEX / HYDROLASE-DNA COMPLEX | ||||||
Function / homology | ![]() pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Birdsall, D.L. / McPherson, A. | ||||||
![]() | ![]() Title: Crystal structure disposition of thymidylic acid tetramer in complex with ribonuclease A. Authors: Birdsall, D.L. / McPherson, A. #1: ![]() Title: Comparison of Two Independently Refined Models of Ribonuclease A Authors: Wlodawer, A. / Borkakoti, N. / Moss, D.S. / Howlin, B. #2: ![]() Title: Nuclear Magnetic Resonance and Neutron Diffraction Studies of the Complex of Ribonuclease A with Uridine Vanadate, a Transition-State Analogue Authors: Borah, B. / Chen, C.-W. / Egan, W. / Miller, M. / Wlodawer, A. / Cohen, J.S. #3: ![]() Title: Active Site of RNase: Neutron Diffraction Study of a Complex with Uridine Vanadate, a Transition-State Analog Authors: Wlodawer, A. / Miller, M. / Sjolin, L. #4: ![]() Title: Structure of Ribonuclease A: Results of Joint Neutron and X-Ray Refinement at 2.0 Angstroms Resolution Authors: Wlodawer, A. / Sjolin, L. #5: ![]() Title: The Refined Crystal Structure of Ribonuclease A at 2.0 Angstroms Resolution Authors: Wlodawer, A. / Bott, R. / Sjolin, L. #6: ![]() Title: Hydrogen Exchange in RNase A: Neutron Diffraction Study Authors: Wlodawer, A. / Sjolin, L. #7: ![]() Title: Structure of Ribonuclease A: X-Ray and Neutron Refinement Authors: Wlodawer, A. / Bott, R. / Sjolin, L. #8: ![]() Title: Joint Refinement of Macromolecular Structures with X-Ray and Neutron Single- Crystal Diffraction Data Authors: Wlodawer, A. / Hendrickson, W.A. #9: ![]() Title: Orientation of Histidine Residues in RNase A: Neutron Diffraction Study Authors: Wlodawer, A. / Sjolin, L. #10: ![]() Title: Studies of Ribonuclease A by X-Ray and Neutron Diffraction Authors: Wlodawer, A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 31.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 25 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 439.5 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 11.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Atom site foot note | 1: RESIDUES 93 AND 114 ARE CIS PROLINES. |
-
Components
#1: DNA chain | Mass: 1171.814 Da / Num. of mol.: 1 / Source method: obtained synthetically |
---|---|
#2: Protein | Mass: 13708.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.12 % |
---|---|
Crystal grow | Details: THE COMPLEX WAS FORMED BY DIFFUSION OF DNA INTO THE NATIVE CRYSTALS. |
-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2.5 Å / Num. obs: 4466 / Observed criterion σ(I): 2 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Rfactor Rwork: 0.235 / Rfactor obs: 0.235 / Highest resolution: 2.5 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.5 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 10 Å / Num. reflection obs: 4218 / Rfactor obs: 0.235 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 5.4 |